William S. Klug Michael R. Cummings Charlotte A. Spencer Concepts of Genetics Eighth Edition Chapter 19 Recombinant DNA Technology Copyright © 2006 Pearson Prentice Hall, Inc.

Recombinant DNA Technology Combines Several Experimental Techniques

Recombinant DNA Technology Is the Foundation of Genome Analysis

Restriction Enzymes Cut DNA at Specific Recognition Sequences

Figure 19-1 Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 19-2 Copyright © 2006 Pearson Prentice Hall, Inc.

11,294 (There are at least this many known restriction enzymes)

Figure 19-3 Copyright © 2006 Pearson Prentice Hall, Inc. palindrome

Figure 19-4 Copyright © 2006 Pearson Prentice Hall, Inc. Not really a gap

Vectors Carry DNA Molecules to Be Cloned Plasmid Vectors

Figure 19-5 Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 19-6 Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 19-7 Copyright © 2006 Pearson Prentice Hall, Inc.

Lambda ( ) Phage Vectors

Figure 19-8 Copyright © 2006 Pearson Prentice Hall, Inc.

Figure 19-9 Copyright © 2006 Pearson Prentice Hall, Inc.

Cosmid Vectors

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Bacterial Artificial Chromosomes

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Expression Vectors

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DNA Was First Cloned in Prokaryotic Host Cells

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Yeast Cells Are Used as Eukaryotic Hosts for Cloning

Table 19-1 Copyright © 2006 Pearson Prentice Hall, Inc.

Figure Copyright © 2006 Pearson Prentice Hall, Inc.

Genes Can Be Transferred to Eukaryotic Cells Plant Cell Hosts

Figure Copyright © 2006 Pearson Prentice Hall, Inc. From Agrobacterium tumifaciens

Mammalian Cell Hosts

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The Polymerase Chain Reaction Makes DNA Copies Without Host Cells

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The Polymerase Chain Reaction Makes DNA Copies Without Host Cells Limitations of PCR Other Applications of PCR

Libraries Are Collections of Cloned Sequences Genomic Libraries Chromosome-Specific Libraries

Figure Copyright © 2006 Pearson Prentice Hall, Inc. Flow cytometry

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Libraries Are Collections of Cloned Sequences cDNA Libraries

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Specific Clones Can Be Recovered from a Library Probes Identify Specific Clones Screening a Library

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Cloned Sequences Can Be Characterized in Several Ways Restriction Mapping

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Nucleic Acid Blotting

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DNA Sequencing Is the Ultimate Way to Characterize a Clone

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Figure Copyright © 2006 Pearson Prentice Hall, Inc. T T C G T G A A 5’-TTCGTGAA…etc

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Figure Copyright © 2006 Pearson Prentice Hall, Inc.

DNA Sequencing and Genome Projects