snRNP (snRNA) nello spliceosoma

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Watson et al. , BIOLOGIA MOLECOLARE DEL GENE, Zanichelli editore S. p

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Presentation transcript:

snRNP (snRNA) nello spliceosoma

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Intermedi di splicing: complessi E, A

Intermedi di splicing: complessi B

Intermedi di splicing: complessi C

Watson et al. , BIOLOGIA MOLECOLARE DEL GENE, Zanichelli editore S. p Watson et al., BIOLOGIA MOLECOLARE DEL GENE, Zanichelli editore S.p.A. Copyright © 2005

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

introne esone …CUGCUGAGGCGAGGUAGAUACA........CUCCUCCAUAGGUGGUGCUGGC... ...UACUGCUGAGGCGAGGUGGUGCUGGGCAUC..

trans-esterificazioni Tipi di splicing meccanismo simile Splicing nucleare Splicing di introni di tipo II Splicing di introni di tipo I Splicing di tRNA di lievito trans-esterificazioni spliceosoma autosplicing nucleasi e ligasi

RNA catalitico RNAsi P Viroidi e virusoidi Introni di gruppo I e II

La “testa di martello” (hammerhead) G C U A 5'ppp 3' OH ribozima G C N A U loop 1 loop 2 loop 3

Ribozima “hammerhead”

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Struttura dell’introne dell’rRNA di Tetrahymena

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Trans-splicing in C. elegans

C. elegans Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Splicing alternativo

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Regolazione dello splicing

Editing

Editing dell’mRNA per la Apolipoproteina B umana

Inosina Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Editing dell’mRNA per la subunità B di GluR

Editing inserzionale in Tripanosoma

Mitocondri tripanosomi Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006

Editing inserzionale mediante RNA guida

Lewin, IL GENE VIII, Zanichelli editore S.p.A. Copyright © 2006