Stomata Lab Leaf fingerprinting

Lab Prep Paint 2 cm by 2 cm square of clear nail polish on top and bottom of leaf

Photosynthesis

Photosynthesis Equation CO 2 + H 2 OC 6 H 12 O 6 + O 2 light 666

Light vs. Dark LocationReactantsProducts Light Reaction Thylakoid Membrane Sunlight H 2 O ATP, NADPH, and oxygen Dark Reaction StromaATP, NADPH, CO 2 Glucose

Stomata Small pores located on the underside of a leaf Allow –CO 2 in –O 2 and H 2 O out

Stomata When are they open? –Day When are they closed? –Night

Guard Cells Swell: Cell’s vacuole fills with water –Stomata Open –Day Shrink: Water leaves cell’s vacuole –Stomata Close –Night

Where would you find stomata on a lily pad? Why?

Microscope Review What is the correct way to hold a microscope? –Two hands! –Support arm and base of microscope! What objective lens do I use to first focus an image? –low

Microscope Review When do you use the coarse adjustment knob? –ONLY ON LOW –NEVERCOARSE ADJUSTMENT –NEVER TOUCH COARSE ADJUSTMENT ON ANY OTHER OBJECTIVES! If I am looking at an image on the 40x objective lens, what is my TOTAL magnification? –40x objective10x ocular400x total magnification –40x objective x 10x ocular = 400x total magnification

Stomata Lab Label: stomata, guard cells, total magnification HIGH Power LOW Power

Stomata Lab FOV (Field of View): # of stomata you can see LOW Power