Pulse Electroporation System Onyx Medical Inc.

Various TDD Technologies Various TDD Technologies  Electroporation - Using short electrical modulated pulses of high voltage to create transient aqueous pores in the skin.  Iontophoresis – Using low voltage electrical current to drive charged drugs through the skin.  Sonophoresis – Using ultrasound to disrupt the stratum corneum.  Thermal energy – Using heat to make the skin more permeable  Magnetophoresis - has been investigated as a means to increase drug flux across the skin.  Needle Free Injection - devices for “firing” molecules through the skin at high velocity

Electroporation  Non-invasive  Alternative to mesotherapy  Virtual mesotherapy or without needles  Painless procedure  Introduction of drugs  alternating current  rearrange the structure in the cells of the outer layer of skin  Acts as a virtual syringe  Changes direction (polarity) continuously slowly  Generating new pores  Micro and macromolecules  500,000 Dalton as collagen.  Dalton as hyaluronic acid

Electroporation  For electroporation we need:  Membrane potential: volts  Disrupt lipid layer  Open biological channels (aquous)  These channels stay open several seconds  Change polarity constantly

When the pulse ceases, the membrane returns to its original structure, leaving retained product that has penetrated into the interior of the cell in place. The application of an electrical pulse momentarily disrupts the cell membrane, allowing entry of product. Background

Electroporation  Electric doors called biological channels  Electric pulse changes membrane polarity  By slowly changing the polarization we do not cause electrolysis  This disrupts lipid layer

Open Channels

Intercellular Communications

Cellular Penetration

Electroporation  This effect lasts for seconds, therefore allowing the introduction of substances.  Hydrofilic and hydrophobic molecules penetrate at the rate of 1 g every minutes.  Continuous formation and appearance of biological channels  Dramatically increases skin permeability.

Conclusion  Electroporation is a process that uses high-voltage current to make cell membranes permeable to allow the introduction of new materials.  In a practical sense Electroporation is opening a door that allows us to insert “products” into the body.

Methodology  Research documents 4 times better results when you do an exfoliation such as a peel or micromicrodermabrasion before Electroporation.  This eliminates dead cells from the Keratinic layer.  This changes the skin potential and makes it easier to create biological channels.

Comparison with Microdermabration

Indications  Introduction of substances for: –Skin treatment –Cellulite –Flaccidity –Wrinkles –Obesity –Collagen production

Contraindicaciones  Pacemakers, heart valves or other active implants.  Epilepsy  Pregnant women   Immediately after Botox  Skin outbreaks or on infected tissue  Metal plates or other metal objects  Ulcer or gastroenteritis

Wrinkles Instruction: Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm. Select Protocol Power:70% - 80% Time of application: 20 – 25 minutes Amount of product: 4 to 6 cc, divided into face, neck, and décolleté Method: Circular motion, soft and slow until completely absorbed Frequency of session: One session a week during five to six weeks After one session every two week for one month A monthly session as maintenance

Decolette Instruction: Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm. Select Protocol Power: 70% - 80% Time of application : 15 – 20 minutes Amount of product :4 cc Method: Circular motion, soft and slow until completely absorbed Frequency of session: One session a week during five to six weeks After one session every two week for one month A monthly session as maintenance

Body Firming Instruction: Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm. Select Protocol Power: 80% - 90% Time of application: 15 – 20 minutes Amount of product: 5 to 6 cc (depending if we have to reduce or reaffirm) Method: Circular motion, soft and slow until completely absorbed Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

Breast Firming Instruction: Clean and dry skin the skin. Exfoliate by microdermabrasion or gentle enzyme peel. Select Protocol Power: 80% - 90% Time of application: 15 – 20 minutes Amount of product:3 - 4 cc Method: Circular motion, soft and slow until completely absorbed Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

Facial Rejuvenation Instruction: Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm. Select Protocol Power: 70% + Face, 30-40% Lips Time of application: 20 – 25 minutes Amount of product: 5 to 6 cc, divided into face, neck, and chest (also hands) Method: Circular motion, soft and slow until absorbed Frequency of session: One session a week for three to four weeks After one session every two week for one month A monthly session as maintenance

Localized Obesity Instruction: Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm. Select Protocol Power: 85% - 95% Time of application: 15 – 20 minutes Amount of product: 6cc - 10 cc Method: Circular motion, soft and slow until absorbed Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

Cellulite Instruction: Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm. Select Protocol Power: 85% - 95% Time of application: 15 – 20 minutes Amount of product: cc Method: Circular motion, soft and slow until absorbed Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance

ELECTROPORATION ONYX MEDICAL INC WWW.ONYXMEDICAL.COM