PROTEIN SYNTHESIS BY: MARIAH GUMFORY. OBJECTIVES Explain the purpose and process of transcription and translation Recognize that gene expression is a.

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PROTEIN SYNTHESIS BY: MARIAH GUMFORY

OBJECTIVES Explain the purpose and process of transcription and translation Recognize that gene expression is a regulated process Describe the roles of DNA and RNA in cell differentiation Identify and illustrate changes in DNA and evaluate the significance of these changes

PROTEIN SYNTHESIS VIDEO

WHAT IS PROTEIN SYNTHESIS? Process whereby DNA encodes for the production of amino acids and proteins Refers to a multi-step process, beginning with amino acid synthesis and transcription of nuclear DNA into messenger RNA, which is then used as input for translation

WHY DO WE NEED IT? We need proteins! Proteins make up enzymes, hormones and components of cell membranes among many other things

MRNA Molecule of RNA that encodes a chemical "blueprint" for a protein product Carries coding information to the sites of protein synthesis: the ribosomes Read in a series of triplets called codons  Example of a codon: AUG

TRNA Functions as an interpreter between nucleic acid and peptide sequences by picking up amino acids and matching them to the proper codons in mRNA Each tRNA carries one amino acid that corresponds to an mRNA codon The proper amino acid is joined to the tRNA by the enzyme aminoacyl-tRNA synthetase

RIBOSOME Composed of two subunits: a large and a small Work bench for protein assembly

STEP 1: TRANSCRIPTION DNA unwinds and “unzips” Enzymes match RNA nucleotides to unzipped nitrogen bases of the DNA and form one strand of mRNA The strand of mRNA detaches and heads off out of the nucleus for a ribosome DNA re-zips and twists back up again

STEP 2: TRANSLATION Small ribosomal subunit binds to the mRNA Initiator tRNA binds to the binds with the start codon of mRNA Large ribosomal subunit binds to the complex and starts the polypeptide chain A second tRNA comes in and binds to the next mRNA codon The ribosome advances down the line to the next codon, and the process continues Rinse and repeat until a STOP codon is reached and a brand new polypeptide is released and ready to be used.

DRAG AND DROP ACTIVITY tm

OBJECTIVES Explain the purpose and process of transcription and translation Recognize that gene expression is a regulated process Describe the roles of DNA and RNA in cell differentiation Identify and illustrate changes in DNA and evaluate the significance of these changes