Microscopes

What is this?

I. Parts of the Compound Light Microscope Metal or plastic part on which microscope rests BASE

Somewhat C-shaped pillar arising from the base and supports the stage and ocular components. Used for carrying ARM

Flat platform attached to lower portion of arm on which slides or samples are STAGE

Collects light rays and focuses them on the specimen slide Collects light rays and focuses them on the specimen slide. Can be adjusted for better resolution (sharper image) DIAPHRAGM

Convex lens used to magnify the image; generally 10-15x magnification Ocular or eyepiece

Small convex lenses screwed into nosepiece and increase the magnification of the sample. Low power = 4x high power =40x OBJECTIVES

COARSE ADJUSTMENT KNOB Larger knob which moves the base rapidly up or down to get the sample into coarse focus COARSE ADJUSTMENT KNOB

Smaller knob which moves the base slowly through short distances to get the sample into sharp focus FINE ADJUSTMENT KNOB

REMEMBER: All specimens must be thin for LIGHT to shine through them so that you can see clearly.

REMEMBER Only use the FINE ADJUSTMENT KNOB when focusing on medium or high power objectives!! This avoids damaging or breaking the slide

Electron microscope Electron microscope – a microscope that focuses beams of electrons instead of light rays. Produces clearer, 3D images.

Muscle Tissue!

LIGHT MICROSCOPE VS. ELECTRON MICROSCOPE I. USED TO VIEW LIVING OBJECTS I. OBJECTS CANNOT BE ALIVE II. COLOR AND MOVEMENT CAN BE OBSERVED II. BLACK AND WHITE IMAGES WITH NO MOVEMENT III. POOR RESOLUTION III. EXCELLENT RESOLUTION IV. EASY TO USE/INEXPENSIVE IV. VIEWER MUST BE TRAINED/VERY EXPENSIVE

Calculating magnification Just multiply the objective lens power by the ocular lens power Lower power: 10 X 4= 40 Medium power: 10 x10= 100 High power: 10x40=400

Guided Practice Instructions: You will be using your fingers to show me the correct answer for the following questions We are going to read the question SILENTLY. DO NOT CALL OUT THE ANSWER. Think about the question and pick an answer. When I count to 3, You are going to hold up your choice. DO NOT LOOK AT ANYONE ELSE’S ANSWER!! THIS IS YOUR TIME TO FIGURE OUT IF YOU KNOW THE INFORMATION!

QUESTION 1 1. One advantage of using a light microscope is that it – A. has high resolution B. magnifies clearly to 1000x C. can be used to view living cells D. can be used to view atoms

QUESTION 1 1. One advantage of using a light microscope is that it – A. has high resolution B. magnifies clearly to 1000x C. can be used to view living cells D. can be used to view atoms

QUESTION 2 2. When you are focusing on medium or high power objectives, you should use the Coarse knob adjustment Fine knob adjustment Ocular Diaphargm

QUESTION 2 2. When you are focusing on medium or high power objectives, you should use the Coarse knob adjustment Fine knob adjustment Ocular Diaphargm

Question 3 The purpose of the diaphragm is to Adjust the amount of light focusing on the specimen Magnify the specimen on the stage Adjust the magnification of the specimen View the specimen on the stage

Question 3 The purpose of the diaphragm is to Adjust the amount of light focusing on the specimen Magnify the specimen on the stage Adjust the magnification of the specimen View the specimen on the stage

Question 4 If a microscope’s ocular is marked 10x and the low power objective is marked 4x, what would be the total magnification when using the low power objective? A. 10x C. 40x B. 12x D. 400x

Question 4 If a microscope’s ocular is marked 10x and the low power objective is marked 4x, what would be the total magnification when using the low power objective? A. 10x C. 40x B. 12x D. 400x

Question 5 If a microscope’s ocular is marked 10x and the low power objective is marked 30x, what would be the total magnification when using the low power objective? 3x 300x 30x 3000x

Question 5 If a microscope’s ocular is marked 10x and the low power objective is marked 30x, what would be the total magnification when using the low power objective? 3x 300x 30x 3000x

Question 6 One disadvantage of using an electron microscope is that it A. has high resolution B. Very expensive C. shows details of the surface D. can be used to view atoms

Question 6 One disadvantage of using an electron microscope is that it A. has high resolution B. Very expensive C. shows details of the surface D. can be used to view atoms

Worktime How should you carry a microscope? What knob should you use to focus when using a high power objective? Why should you not use the coarse knob adjustment when using the high power objective?

Worktime What is the purpose of the diaphragm? How should you carry a microscope? What knob should you use to focus when using a high power objective? Why should you not use the coarse knob adjustment when using the high power objective? What is the purpose of the diaphragm? Where is the ocular lens located? Where are the objective lens located What is the purpose of the stage? If the ocular lens has a magnification of 10x and the objective lens has a magnification of 25x, what is the total magnification? If the ocular lens has a magnification of 4x and the objective lens has a magnification of 50x, what is the total magnification? If the ocular lens has a magnification of 5x and the objective lens has a magnification of 100x, what is the total magnification?

Worktime How should you carry a microscope? What knob should you use to focus when using a high power objective? Why should you not use the coarse knob adjustment when using the high power objective? What is the purpose of the diaphragm? Where is the ocular lens located? Where are the objective lens located What is the purpose of the stage? If the ocular lens has a magnification of 10x and the objective lens has a magnification of 25x, what is the total magnification? If the ocular lens has a magnification of 4x and the objective lens has a magnification of 50x, what is the total magnification? If the ocular lens has a magnification of 5x and the objective lens has a magnification of 100x, what is the total magnification?

Exit Ticket:1 I used to work at an Air force base. We would work with particles of metal called nano particles. The particles were dead, and microscopic. What would be the best way to see those? A light microscope An electron scanning microscope A light telescope An electron scanning teloscope

Exit Ticket 2: 2) When the low power objective is being used you should adjust it using the: a) fine adjustment knob b) diaphragm c) course adjustment knob d) ocular adjustment knob

Exit Ticket 3 3) Which of the following statements is NOT true about objects viewed with an electron scanning microscope? A. They must be thin enough to allow light to pass through them. B. They must be living specimens C. No dyes or states can be used on the objects D. They must be made of organic material

Exit Ticket 4 4) If the microscopes ocular lens is marked 10x and the lower power objective is marked 5x, what is the total magnification? a) 10 b) 5 c) 50 d) 500

Exit Ticket: 5 5) What is the proper way to place a cover slip on a wet mount slide? a) c) b) d)