Image-Pro Premier Basic Training Course Part 4 - Calibration and Measurements

Measuring Objects Groups of pixels together make objects From Outlines we can get measurements: Perimeter Area Bounding box Centre coordinates Radii Much, much more...

Calibrations Calibrations make sense of measurement Transforms distances from Pixels to real world units All images must be calibrated for spatial measurements 40µm

Creating Calibrations Use known values to estimate Cal = Sampling Rate Instrument Mag Or, take image of reference object

Creating Calibration 1. Take image of known sample 2. Go to Capture Ribbon 3. Select Quick Calibration tool 4. Drag line over known distance 5. Select units and qualify line

Calibrating Images From Capture Ribbon Select Calibration from Characteristics box New automatically assume current charactristics calibrations Use green tick mark next to Spatial drop-down for image with no or incorrect calibration

Scale Bars Calibrations enable Scale Bars and Measurements Enable Scale Bars from Calibration group Use Auto Marker to apply markers to every image Use Calibration drop-down to access Marker properties 1mm1mm

Manual Measurements Use Measure ribbon for manual measurements Use Types button to select what values to collect Use drop-downs for additional tools (e.g. straight line Vs poly-line)

Display Option Use Options button to change: Outline styles Label option

Display Option Show statistics in Measurement table Use groupings to arrange object data by class Change grouping options to display class statistics

Exporting Data Export Images using: Quick Save in Capture ribbon Share ribbon Export any data table to Excel, Text files or Clipboard

Measurements Manual Measurements are a powerful tool for getting information from an image but......Manual measurements are also: slow & tedious open to operator bias