Heterotrophic Plate Count Test. 1. Label the 5 Agar Plates: Control, and the names of the four water samples to be tested. (ie. Urban Treated, Rural Treated,

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Presentation transcript:

Heterotrophic Plate Count Test

1. Label the 5 Agar Plates: Control, and the names of the four water samples to be tested. (ie. Urban Treated, Rural Treated, Untreated Raw, Local Treated) 2. Label the 5 sterile pipettes: Control (CW), and the appropriate water sample names (ie. Urban Treated, Rural Treated, Untreated Raw, Local Treated) 3. Label the 4 Heterotrophic Sampling Tubes as follows: Urban Treated, Rural Treated, Untreated Raw, Local Treated. Fill the tubes to the 5 mL mark with the corresponding water sample using the corresponding pipette

3. (Continued) (shake to mix). The Heterotrophic Sampling tube contains 10% Sodium Thiosulphate which removes chlorine from the water sample – the chlorine is what helps keep bacteria from forming in our water. By removing the chlorine from the water sample, colonies should form on the plate.

4. Using the pipette labeled Urban Treated Water, drip 2 drops of the Urban Treated water directly out of the labeled water bottle into the agar in the Control Agar Plate.

5. With a spreader, use a back and forth motion to spread the Urban Treated water sample over the plate. Turn the Agar Plate a quarter turn and continue the back and forth motion to spread the sample. Continue turning and spreading until all the liquid has been absorbed into the plate. Be sure to spread the sample on the surface of the agar, and not to “dig into” the agar and make the agar all chopped up. 6. Repeat steps 4 & 5 using the water samples from the remaining Heterotrophic Sampling Tubes to put on the corresponding agar plates. Remember to use like labeled pipettes with like labeled water samples.

7. Replace the lid, and let them sit for 10 minutes. 8. Flip the plates over, Agar side up, and store plates at room temperature for 7 days.

9. After 7 days the colonies of bacteria can be counted on the Sample Agar Plate. Each colony looks like a small round dot on top of the agar and will vary in size, shape and colour. The Control Agar Plate should not have any colonies. 10. Record the number of colonies counted. Results: If the total number of bacteria colonies counted on the Water Sample Agar Plate is under 500, then it meets the Canadian Drinking Water Guidelines.