Water potential Ψ = Ψs+ Ψp

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Presentation transcript:

Water potential Ψ = Ψs+ Ψp Water Potential, Ψ* , describes the likelihood water will diffuse in or out of a cell. Based on two factors: • solute concentration gradient (aka solute potential) • pressure in the cell (aka pressure potential) • water moves from the sol’n with higher water potential to that with lower water potential Ψ = Ψs+ Ψp *greek letter psi

Ψ = Ψs+ Ψp Solute Potential, Ψs (aka solute conc.) • hypertonic Ψs in environs > in cell cell loses water via osmosis • hypotonic Ψs in environs < in cell Turgor pressure, bail, or burst • Here, cell solute potential has increased, thus cell water concentration decreased • Ψ of pure water = 0, so Ψs is always negative Solute Potential, Ψs (aka solute conc.) Ψ = Ψs+ Ψp cell environs

Pressure Potential, Ψp Ψ = Ψs+ Ψp • if pressure in cell increases, so does Ψ • living cells usually have positive Ψp (turgid, not flaccid) - pressure 0 pressure + pressure

Sum of solute, pressure potentials yields water potential No solute, no pressure Net diffusion into cell No net diffusion

Again: (aka solute potential) At 75, no pressure, but great solute gradient = net diffusion into cell Negative water potential At 100, pressure potential prevents continued solute diffusion despite solute potential = no net diffusion Zero water potential

To quantify Ψ find solute potential: Ψs = -iCRT temperature K = C + 273 pressure constant .0831 L bars/moles K ionization factor sucrose does not ionize in water i = 1.0 molar concentration moles/L Ex: solute potential of a 1.0M sucrose sol’n at 22C at : std atmospheric pressure Ψs = -(1)(1.0)(.0831)(295) Ψs = -24.51bars Ψ = -24.51bars + Ψp Ψ = -24.51bars Since water at atmospheric pressure has a pressure potential of zero, water pot. = solute pot. (+ 0)