Developing a dye using Caesalpinia sappan (Pathangi) wood extract BT 3064 (Experimental Plant Biotechnology) Department of Plant Sciences

BT 3064 (Experimental Plant Biotechnology) course module Level III Plant Biotechnology (Special Degree) Group work of 6 students Supervised by, K.P.D. Tharaka W.A.K.T.M. Anandawansha P.N.A. Dayananda P.V.D. Dilani U.W.R. Sampath D.I.M. Yahampath Dr. Sudeera Ranwala Dr. Pradeepika Saputhanthri

What are natural dyes Dyes derived from natural sources Dyes are coloured, ionizing and aromatic organic compounds which show an affinity towards the substrate to which it is being applied ( Nemcova et al, 2014)

Why use natural dyes? Provide an environment friendly dye for fabrics and garments Do not contain chemicals harmful to health and more reliable than the chemical synthetic dyes Reducing production costs and eliminating the huge expenses of chemical imports Biodegradable and do not cause disposal problems

Pathangi (Caesalpinia sappan) as a natural dye The heartwood of the plant is used as dyeing agent The main dye component in the heartwood is brazilin Brazilin is a weakly coloured product Easily oxidises by air and light to give the deep red pigment brazilein.

Our tasks : Aims and objectives To improve the extraction protocol by testing the effectiveness of different solvents. To investigate the effect of incorporating Kora-Kaha (Memecyclon umbellatum ) leaves in the extraction procedure. To find out most appropriate dye amount and mordanting method for effective dyeing of fabrics.

Our tasks : Aims and objectives cont.. To check the effectiveness of the dye, after application through certain techniques such as wash fastness, rub fastness, and photo degradation effect. To check for microbial contaminations after application of dye to a cotton fabric. To develop Pathangi crude as a paint for cellulose papers and to obtain several different colours using different mordants.

Each student conducted standard methodologies Student 1- Extraction of the dye from the wood of Pathangi using different extraction methods Student 2- Investigate the effect of incorporating Kora-Kaha leaves in the extraction procedure Student 3- Determination of photo degradation effect

Student 4- Mordanting of clothes, dyeing of Student 4- Mordanting of clothes, dyeing of mordant clothes and solubility tests for the crude extracts Student 5- Determine the growth of microbes after been applied to clothes Student 6- Development of the crude extract (concentrated) as paint

Results Extraction of the dye using different solvents. - Best solvent- methanol Preparation of the crude of Pathangi

Determination of photo degradation. Liquid extractions After applied to clothes Colour degradation highest- water extraction Lowest- methanol extraction

Mordanting of clothes Best mordant- a mixture of alum (Potassium Aluminium Sulphate) & cream of tartar (Potassium hydrogen tartrate) Colour fainting has been minimized using mordants Non mordent clothes Mordent clothes

Discolouration and visible signs of microbial growth No colonies or discolouration patches on dyed clothes Development of the Pathangi crude as paint. Different colours were obtained using different mordants Mordant used Colour of the crude. Alum Maroon/deep red CuSO4 Dark Brown FeSO4 Brown

What we have gained..?? Group Work How to work as a team How to face challenges Work within given time duration How to deal with others

Acknowledgement Our supervisors, Dr.S.Ranwala and Dr.P.S.Saputhanthri, senior lecturers, Department of Plant Sciences All other lecturers, demonstrators & non academic staff of Department of Plant Sciences Biodiversity secretariat - Wickramasinghepura, Athul Kotte Our dear parents All of our colleagues

Thank You