Alex Adams, Greer Beaumont, Candace Booker, Mark Farrington, Nick Molligan, Robert Power Advisors: Melissa Eskridge and Todd Monroe 1

Clientele Information  Dr. Tiersch, Amy Guitreau, and the employees at the Aqua-Cultural Research Station lab  Customer base  ARS Lab  Fishing farms  Biological research labs 2

Project Decomposition 3

Background  Cryopreservation  Slow programmable freezing  Example: 4

Problem Statement  Our team is attempting to create an inexpensive, freezing device and, in doing so, achieve a cooling rate at which straws containing sperm cells can efficiently be frozen. 5

Melissa’s objective: Our objective: 6

Project Goals & Measurable Objectives  Simplified system modeled after computer controlled freezing system  Complete temperature profile of the system  Utilize Arduino to control subunits: sensory, display, and data logger  Target cooling rate: -4°C/min  Ideal temperature: (-80°C) – (-100°C)  Ideal straw transit time: 20 minutes  Straw throughput: 50/run 7

Concept Generation Arduino:  Mrs. Dugas’ classes  Data organization  Intro to Arduino  Code  Circuit design  Research of materials and Arduino components 8

Materials Temperature Profiling:  Type T Thermocouples  Liquid Nitrogen  Styrofoam box  Mesh grid  galvanized steel Arduino:  Uno (2) (Microcontroller)  LCD Screen Shield  Plastic Breadboard  Jumper Wires  Counting Sensor  SD Card  Data Logger Shield  Thermocouple Shield  Barcode Scanner 9

Constraints Time Liquid Nitrogen Evaporation Data Logger % Error Arduino Compatibility Arduino circuitry that works at low temperatures Range of inputs 10

Design Slide 11

12 Microcontroller Design Matrices BBBArduino UNO Arduino DUE Arduino Yun MintduinoGalileoRaspberry Pi Analog pins Digital pins Memory N/A2KB96KB2.5KB2KB512KBN/A Adequate Research Yes NoYes Cost ParametersOptions

13 MAX31855Multiplexer Price$82.95$44.50 Port Count48 Probe InputCurrent Probes Compatable Require New Probe Purchase Output Resolution 0.25 C VoltageBuilt-I n Voltage translation 3.3V or 5V

Arduino Parameters  Inter Integrated communication (I^2C) 14

Parameters Testing Conditions: A/C 21.1°C Room temperature ~21.3°C Styrofoam box dimensions: Height: 16.5 in./7 in. Width: 15 in./11.75 in. Galvanized steel grid: Lies.932 inches in the box 15.5 x 15.5 in² Liquid Nitrogen: 4 inches (~6 L) Temperature Logger Calibration: See Appendix A 15

Arduino Challenges CHALLENGES:  Compatible Arduino components  3 thermocouples (displayed information)  Bar code scanner  Counter  SD reader (  LCD  Range of inputs SOLUTIONS:  Researching materials (model selection)  Project box (protection from temp.) 16

Design Tools: Structure/Function Chart 17

Gantt Chart – Progress and current location 18

Budget MaterialsQuantityCost Type T Thermocouples15$5-8 per thermocouple Barcode Scanner1$30-40 Arduino DUE Microcontroller1$49.95 Arduino UNO Microcontroller1$29.95 SD Memory Card1$11.99 Counting Sensor1$20-30 LCD Display Screen1$23.99 Chicken Wire1$15.00 Thermocouple Shield1$46.50 Data Logger Shield1$19.95 LCD Screen Shield1$21.95 Counting Sensor Shield1$20-30 Total Cost: $ $  Outside Funding  Grant 19

Thank you! Acknowledgements:  Dr. Todd Monroe  Melissa Eskridge  Amy Guitreau  Dr. Tiersch and the ARS employees Questions? 20

Appendix A  Liquid mercury thermometer and 15 Type T thermocouples  Calibrated using:  Room temperature water (Table 1)  Boiling water  Ice water  Loggers collected 5 minutes of data  Recorded data variation connects with specified % error of the temperature logger 21

Results of Temp. Profiling Numbers & graph in-the-making: 22

Temperature Profiling - Systematic technique - Mesh grid - Notation system - Calibrating - Devising our technique - Measuring - Straw stabilization device - 15 minutes - 3 different settings - DT 300 software 23

Straw Method Pictures Individual Straw Method Straw Stabilization Method 24