© ChromaCon AG // www.chromacon.ch // the CaptureSMB ® process principle 1 Contichrom ® animation - the CaptureSMB ® process principle -

Slides:



Advertisements
Similar presentations
Chromatography Components stationary phase (eg., solid matrix) mobile phase (eg., solvent) solute Solutes which interact differently with the stationary.
Advertisements

© ChromaCon AG // // Convert batch to MCSGP // ver. May Contichrom ® case study: How to convert a batch chromatography process.
Gas Chromatography Introduction 1.) Gas Chromatography
Production of Insulin Reverse Phase – High Pressure Liquid Chromatography Unit (RP-HPLC) Presented by:Justin McComb Rachelle Bolton Young Chang.
T h e G a s L a w s. T H E G A S L A W S z B o y l e ‘ s L a w z D a l t o n ‘ s L a w z C h a r l e s ‘ L a w z T h e C o m b i n e d G a s L a w z B.
PURIFICATION OF GFP USING HIC CHROMATOGRAPHY. Chromatography  A technique used to separate molecules based on how they tend to cling to or dissolve in.
The amazing ability of continuous chromatography to adapt to a moving environment. Roger-Marc Nicoud, Founder of Novasep Barcelona, October 2013.
Anion Exchanger Kristen Galea. Where Are We With in the Process? Entering stream is concentrated with, insulin ester, and denatured proteins This separation.
HPLC Analysis of Ionic Compounds Nicholas H. Snow Seton Hall University.
ION EXCHANGE CHROMATOGRAPHY PREPARED BY- MD.MARUF HASSAN.
LABORATORY 6 PART B PURIFICATION OF M FP FROM AN OVERNIGHT CULTURE.
Introduction to Chromatographic Separations Due to lack of analytical specificity, separations are often necessary Chromatography is about separations.
Multicolumn Continuous Countercurrent Chromatography
IEX Chromatography Presented by: Nikki Apostolakis Helen So Tiffany Yu CHEE450: Engineering Biology.
Expanded Bed ID:M Student : 郭育秀 2007/01/08.
Gel filtration Chromatography
Gas Chromatography By Naaimat Muhammed. Gas- liquid chromatography is based upon the partition of the analyte between a gaseous mobile phase and a liquid.
Which of the following amino acids would be first to elute at pH 7 from an anion-exchange column? a). glutamic acid b). alanine c). lysine d). asparagine.
© ChromaCon AG // // the MCSGP process principle 1 Contichrom ® animation - the MCSGP process principle -
Protein Purification. Why purify Proteins? Characterize Function Activity Structure Study protein regulation and protein interactions Use in assays Produce.
Ion Exchange Chromatography. Cation exchangers They contain immobilized anionic groups that bind to cations. e.g. a matrix with carboxymethyl group(CM)
Cation Exchange Column CHEE450 Leslie Davis. Cation Exchange Following removal of biomass – processes supernatant Separate insulin precursor from glucose,
B IOCHEMICAL INSTRUMENTAL ANALYSIS -11 Dr. Maha Al-Sedik.
Platform downstream processes in the age of continuous chromatography: A case study Mark Brower BioProcess Technology & Expression Bioprocess Development.
Contichrom ® animation - the MCSGP process principle -
High Performance Liquid Chromatography
Continuous Antibody Capture with Protein A Countercurrent Tangential Chromatography: A New Column-Free Approach for Antibody Purification Andrew.
Chromatography Chromatographic separation is based on distribution of separated compound (analyte) between mobile phase and stationary phase Richard Vytášek.
Field Methods of Monitoring Aquatic Systems Unit 9 – Ion-Chromatography Copyright © 2006 by DBS.
PRODUCTION OF HIGHLY PURIFIED BOVINE TRANSFERRIN Group 5B Arjun Goel, Connie Marcelli, Lianne Kark, Sara Dang, Vivien Tai Faculty Advisor Prof C.A. Mims.
Simplicity is the key Continuous purification of monoclonal antibodies L. Landric-Burtin Head of Downstream Processing Development, France Integrated Continuous.
PROTEIN PURIFICATION AND ANALYSIS. Assays Need measures for the object (enzyme activity, chromophore, etc.) and for total protein concentration:
COLUMN CHROMATOGRAPHY
CHROMATOGRAPHY. A process to separate molecules to purify them.
Scavengers and Decomposers Grade 7 Science. Goal  I can… explain the importance of scavengers and decomposers within an ecosystem.
Artificially managed food production Animals don’t turn all the food they eat into new animal. Some of the food is not digested and is lost as waste. Some.
Chapter 28 High Performance Liquid Chromatography.
Adsorption Chromatography 1Dr. Nikhat Siddiqi. Adsorption chromatography refers to the use of a stationary phase or support such an ion-exchange resin,
Biochemical instrumental analysis - 11 Dr. Maha Al-Sedik 2015 CLS 332.
Ion Exchange Design Supervised by: Prof. M. Fahim Done by: Samiya Saqer.
HPLC.
Evaluation of a New Arsenic Adsorbing Resin (WASP) Dr. Robert Stanforth, Ng Aik Teng and Woo Su-Fang National University of Singapore July 2002.
HPLC 1. Introduction 1.Introduction  INSTUMENTAL ANALYSIS  PRACTICAL 213 PHC  HPLC.
Chromatography.
Radioimmunoassay (RIA). RIA Purpose is to determine the concentration of an antigen in solution Competitive binding assay Originally developed by Yalow.
$200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200 $400 $600 $800 $1000 $200.
CHROMATOGRAPHY 1Biochemistry of Medics. Chromatography 2Biochemistry of Medics.
Purification Of Proteins.
Ion Exchange Chromatography
Holocaust Reflection Assignment
Lab 7 – Purification of RFP protein (mFP) from an overnight culture
PowerPoint Presentation BATCH :- 03.
Business English
Business English
DNA Extraction and Purification
Gas Chromatography By Naaimat Muhammed.
Dnyanasadhana College, Thane. Department of Chemistry M. Sc
The ocean supports a variety of plant and animal life.
Prince William Sound. Prince William Sound Transporting and treating oiled birds.
Affinity Chromatography
Purification of recombinant T7 RNA polymerase
Intro to Chromatography CHM 480 Suroviec
Producer-An organism that can make its own food.
The CaptureSMB process principle
Last class Salting out Dialysis Paper 1 discussion.
Principle of separation of different components:
THE PHOSPHORUS CYCLE.
Gel-filtration chromatography of the active fractions from the Mono-Q column marked by the horizontal bar in Fig. 1. Gel-filtration chromatography of the.
Experiment 3 Separation of proteins by Anion exchange chromatography
ආහාර සඳහා වු යහපත් නිෂ්පාදන පිළිවෙත් අවශ්‍යතා
Presentation transcript:

© ChromaCon AG // // the CaptureSMB ® process principle 1 Contichrom ® animation - the CaptureSMB ® process principle -

© ChromaCon AG // // the CaptureSMB ® process principle 2 Contichrom ® & CaptureSMB ® explained

© ChromaCon AG // // the CaptureSMB ® process principle 3 Contichrom ® & CaptureSMB ® explained

© ChromaCon AG // // the CaptureSMB ® process principle 4 Contichrom ® & CaptureSMB ® explained Feed column length Internal concentration t1t1 t2t2 t3t3 Feed Wasted resin capacity in batch chromatography

© ChromaCon AG // // the CaptureSMB ® process principle 5 Feed Contichrom ® & CaptureSMB ® explained Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 6 Feed Contichrom ® & CaptureSMB ® explained Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 7 Wash Contichrom ® & CaptureSMB ® explained Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 8 Elution Contichrom ® & CaptureSMB ® explained Feed Product Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 9 Contichrom ® & CaptureSMB ® explained Feed CIP & Re-equilibration Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 10 Contichrom ® & CaptureSMB ® explained Feed Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 11 Contichrom ® & CaptureSMB ® explained Wash Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 12 Contichrom ® & CaptureSMB ® explained Elution Product Feed Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

© ChromaCon AG // // the CaptureSMB ® process principle 13 Contichrom ® & CaptureSMB ® explained CIP & Re-equil. Feed Start over Step 1: Feed & wash Step 2: elute product feed column Step 3: CIP & re-equil. feed column

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.