Identifying research resources should be easy Anita Bandrowski, representing the RII team

A SYSTEM TO IDENTIFY NOT JUST WHO PRODUCED A FINDING, BUT WHAT PRODUCED IT Faulty Antibodies Continue to Enter US and European Markets, Warns Top Clinical Chemistry Researcher- Genome Web Daily, October 11, 2013 “…of the findings in the literature about neuronal NF-κB are based on data garnered with antibodies that are not selective for the NF-κB …” --Herkenham et al. “…of the findings in the literature about neuronal NF-κB are based on data garnered with antibodies that are not selective for the NF-κB …” --Herkenham et al.

W HAT STUDIES USED MY MONOCLONAL MOUSE ANTIBODY AGAINST ACTIN IN HUMANS ? The following antibodies were used for immunoblotting: -actin mAb (1:10,000 dilution, Sigma-Aldrich); - tubulin mAb (1:10,000, Abcam); T46 mAb (specific to tau 404–441, 1:1000, Invitrogen); Tau-5 mAb (human tau 218–225, 1:1000, BD Biosciences) (Porzig et al., 2007); AT8 mAb (phospho-tau Ser199, Ser202, and Thr205, 1:500, Innogenetics); PHF-1 mAb (phospho-tau Ser396 and Ser404, 1:250, gift from P. Davies); 12E8 mAb (phospho-tau Ser262 and Ser356, 1:1000, gift from P. Seubert); NMDA receptors 2A, 2B and 2D goat pAbs (C terminus, 1:1000, Santa Cruz Biotechnology)… mAb=monoclonal antibody

… SURELY THIS YOU HAVE FOUND A TERRIBLE PAPER, THIS CAN ’ T BE THE NORM

Hypothesis: Resources in the published literature are not uniquely identifiable Gather journal articles 5 domains: Immunology Cell biology Neuroscience Developmental biology General biology 5 domains: Immunology Cell biology Neuroscience Developmental biology General biology 3 impact factors: High Medium Low 3 impact factors: High Medium Low 84 Journals 238 papers 707 antibodies 104 cell lines 258 constructs 210 knockdown reagents 437 model organisms Vasilevsky et al, PeerJ, 2013

The problem is general across multiple resource types and disciplines Vasilevsky et al, Peer J 2013

R ESOURCE I DENTIFICATION I NITIATIVE Two pre-meetings with editors and publishers Society for Neuroscience, 2012 NIH: June, 2013 Society for Neuroscience, 2013 Designed pilot project Entities Procedure Infrastructure Established working group through FORCE11 Signed up partners Led by: Matt Brush, Nicole Vasilevsky, Anita Bandrowski And more

P ILOT P ROJECT Authors to identify 3 types of research resources: Software /databases Antibodies Model organisms Include RRID in methods section Voluntary for authors Journals did not have to modify their submission system Journals have flexibility in implementation. Send request to author at: Submission During review After acceptance Launched February 2014: 3 month commitment and more…

RII P ORTAL A single portal for authors >10 databases One search interface Simple directions Big “Cite This” button Uniform format for citation Help desk for authors

W HAT STUDIES USED … >100 articles have appeared to date 15 journals 630 RRID’s 3 removed by typesetting 95% correct 14% false negative rate >200 antibodies were added >75 software tools/databases were added Database available at:

An update of Vasilevsky et al.

W HAT CAN WE DO WITH AN RRID? A resolver service has been created 3rd party tools are being created to provide linkage between resources and papers Utopia prototype ScienceDirect

W HAT HAVE WE LEARNED ? Authors are willing to adopt new types of citations Authors were fairly accurate at performing the task RRID’s resolved by search engines without requiring specialized citation services Citation drives registration Clear role for repositories as authorities

H OW C AN Y OU H ELP ? Authors: Use IDs in YOUR next paper. At least 100 of your friends already have. scicrun.ch/resources Tool Makers: Register your tools! Make authors job easy. Display the proper citation format proudly. Reviewer: Ask authors to put identifiers in their methods, you know they will do almost anything to get you off their back. Editors: Still time to join the RII, go to Force11 to download a sample letter to authors. Publishers: Central instructions to authors have been updated at Springer and Elsevier, where are yours?