Status Report DNA Barcoding in Argentina Pablo Luis Tubaro Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”

iBOL Argentina Fund Five calls for projects from 2008 to 2012, 110 projects supported. The structure of the iBOL project in Argentina iBOL Argentina Committee Five Reference Barcode Labs.Leading Labs Training Workshops for DNA Barcoding. iBOL coordinator Cruises of the Oceanographic vessel

Structure of Argentinian barcoding network MACN – Buenos Aires Nágera Biological Station Mar del Plata INIBIOMA - Bariloche CENPAT – Puerto Madryn IBONE - Corrientes

How Barcode Network works in Argentina Projects supported by iBOL Argentina Fund Projects supported by iBOL Argentina Fund BIO/CCDB Lysis plates PCR Plates (no permits required) Exporting permits (Fauna, Acceso Rec. Genéticos 226/2010) Five Reference Barcode Labs CONICET Llabs. Training Workshops at MACN Barcodes Projects supported by iBOL Argentina Fund Building the DNA barcode library of Argentinian species

The projects supported by iBOL Argentina Fund The researchers must be principal investigators of a PIP or a PICT. Tissues and vouchers must be stored in permanent collections. Tissues and vouchers must be compliant with barcode standards. The funds support only the extra costs of preserving tissues and vouchers, including small equipments, technical services, etc. Only small amounts are allowed to support field work. No PCR and sequencing costs are supported. Researchers and their fellows have to take the Leading Labs Training Workshop for DNA barcoding. Specimens must be collected following legal norms. Researchers have to send the tissue samples to CONICET – iBOL Labs. to produce their barcode sequences.

Leading Labs Training Workshop for DNA Barcoding - MACN

“Puerto Deseado” cruises to get samples for iBOL projects

The iBOL-IDRC project Objetives: Engaging Developing Nations in the International Barode of Life Project (iBOL) To support some key elements of the iBOL project structure. To create barcode libraries that allow practical applications in the participating countries. To secure the active participation of five developing nations in the iBOL project. To develop an ABS policy for the iBOL project.

Actinopterygii United States12810Argentina2184China1364 Australia9033Philippines2130Panama1295 Mexico6527India2048Malaysia1166 Brazil6444Taiwan1680Madagascar885 Canada6292Kenya1642New Caledonia747 Indonesia3520France1607Seychelles740 South Africa2238French Polynesia barcodes belonging to species

Canada4992Brazil1741Bolivia555 Peru3491Japan1399Australia538 Argentina3195Indonesia1109Sweden449 Panama2046Papua New Guinea695Netherlands448 United States1873New Zealand642Philippines336 Mexico1788Norway631Spain318 Russia1756Ecuador612 Aves barcodes belonging to 4563 species

Costa Rica157609Germany12133South Africa6400 Australia106719China10924Panama5823 Canada98213Brazil10141Argentina5466 United States90582Peru9807Finland4519 Papua New Guinea19524Indonesia8028United Kingdom4295 Mexico15256Italy7380Russia4287 Ecuador12961Spain7141 Lepidoptera barcodes belonging to species

Canada2565Thailand463Panama160 Costa Rica1583Brazil453Colombia121 United States1459Bolivia266India114 Argentina743South Africa239Peru111 Kenya688Guatemala232Chile104 Germany678Russia230Norway99 Mexico613Australia185 Apidae barcodes belonging to 2021 species

Barcode Argentine Butterflies (ARLEP) and Moths (ARMOT)

ARMOT

Emerging Leaders in the Americas Program - ELAP PLO0308 AAA % COI divergence 4.7% COI divergence 12.2 COI divergence PLO0104 ACA8897 PLO0104 AAA9669 Finding a deletion

Building the Biodiversity Macroscope in Bolivia

CIAR – Pcía de Misiones – Selva subtropical Ea. El Bagual – Pcia de Formosa – Chaco húmedo Malaise 1: ºS ºO Malaise 2: ºS ªO 139 msnm EQP-CLL ’10.0” S 58 48’54.0”W 57 msnm EQP-CLL ’11.1” S 58 48’55.8”W 56 msnm Global Malaise Program

Future plans (next 12 months) To continue working in collaboration with Bolivia to build its Biodiversity macroscope, overcoming its new regulations. To increase the production of COI products in CONICET’s Barcode Labs. To expand the Global Malaise Trap Program in protected areas in Argentina and Bolivia and set their sample processing pipelines. To help other Latin American countries in barcode projects – e.g. Peru, Dominican Republic, etc. To open a new call of projects for the iBOL Argentina Fund and to organize the Sixth LLABs training workshop for DNA barcoding. Secured Next trip to Bolivia postponed given current restrictions; additional support required to continue after 2013 Working on both aspects, additional funds required

Next biota? The Biodiversity of the Iguazú National Park - Argentina

The Iguazú National Park (in Argentina) and the Iguaçu National Park (in Brazil) represent the southern tip of the Atlantic forest, and together with the preserves of Misiones Province are the largest and best preserved remains of this ecosystem. The Atlantic forest, one of the richest and more endangered ecosystems of the World

Objectives 1)To discover which species are within Iguazú National Park. 2) To build a DNA barcoding database that will permit the identification of the species of Iguazú National Park in a cost-effective and rapid manner. 3) To describe the new species that will result from sampling the fauna of Iguazú National Park. 4) To provide a biodiversity data baseline for the Iguazú National Park that will be a benchmark against which future biodiversity studies can be compared.