All origami shown in this report is from the same annealing run that Massud showed in his 6/21/2011 weekly report

biotin/streptavidin position on A tile of 2D DNA array (see next slide) staple:m13 5:1 [m13]=10 nm SA:biotin staple 15:1 this annealing run was not dialyzed before adding SA and imaging

58.88nm Two biotin on each side of A tile SA labeled A tile

SA labeled 2D cross DNA origami array

A subunit with total 4 biotin B subunit with no biotin + anneal over weekend A subunit with total 4 biotin B subunit with no biotin + 15:1 SA:biotin staples AB array XAB array YSAAFM #1 (mica) AB array X AB array YSA nM SA (15:1) anneal over weekend + 4 deg C overnight AB array XSAAB array YSASA AFM #2 (mica) All AFM images shown in final 6 slides of this presentation were previously shown in group meeting by Massud and are of AB array XSA below is a flowchart type key to the AFM images in the subsequent slides, any images that are not square can be assumed to have long side (X) length equal to the Y range seen on side of image. Unless otherwise stated, all images on a single.ppt slide are of the very SAME mica surface/depostion. Also, unless stated, deposition was by 5 min. incubation of origami soln. on mica followed by single HOH rinse.

AB array YSAAFM #1first mica after one HOH rinse

AB array YSAAFM #1same mica after second HOH rinse

AB array YSASAAFM #2first mica after one HOH rinse

AB array YSASAAFM #2second mica one HOH rinse

AB array XAFM #1

AB array XSAAFM #2 this is the first piece of mica imaged, TAE rinsed at left, TAE rinse followed by HOH rinse below

AB array XSAAFM #2second piece mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; continued next slide

same mica surface/ deposition as seen in previous slide

AB array XSAAFM #2 second peice mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; the only difference between this slide and the 2 preceding slides is that the same mica surface seen in previous 2 slides, sat in covered dish in ambient conditions for 6 days prior to acquisition of the images on this slide

Massud included the following slides in his presentation

SA labeled 2D cross DNA origami array (this and all slides following are of AB array XSA)

SA labeled 2D cross DNA origami array