Kingdom of Saudi Arabia King Saud University College of Applied Medical Sciences Biomedical Technology Department.

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Research Techniques Made Simple: Polymerase Chain Reaction

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Presentation transcript:

Kingdom of Saudi Arabia King Saud University College of Applied Medical Sciences Biomedical Technology Department

By: Badr AlShamary & Mohannad Abuljadayel Supervised By: Mr. Mohammed Nisar

Polymerase Chain Reaction molecular biological method for amplifying DNA without using a living organism. PCR is commonly used in: Identification of genetic fingerprints. Diagnosis of infectious diseases. Diagnosis of virus diseases.

A T G CA TC G The four bases found in DNA are Adenine (A), Cytosine (C), Guanine (G) and Thymine (T).

Thermocycler

Temperature Graph

Sample Contents DNA original Sample Primers DNA Polymerase Buffer Solution Nucleotides(A,T,C and G) Primers DNA Polymerase Buffer Solution Nucleotides

PCR Process Step1: Denaturation

PCR Process Step2: Annealing

PCR Process Step3: Extension

PCR Process Doubling DNA each Cycle (Cycle:2)

PCR Process (Cycle: 3) Number of copies= 3 2 = 8

Virus Diagnosis By PCR Human DNA Virus DNA

Human DNA Virus Diagnosis By PCR Virus DNA

Human DNA Virus Diagnosis By PCR Virus DNA

Thank You