Supplemental Figure 1 A. EpCAM Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the EpCAM specific PE Fluorochrome (488nm laser, 585/42-A emission filter) (x axis; y axis = CD45 PE Cy7, 488 nm laser, 780/60-A emission filter). The corresponding unstained cytometry enumeration gate displayed no EpCAM positive populations. Bottom Row: Histogram showing EpCAM Antibody stained BT474 cells with high fluorescent intensity in the PE Fluorochrome channel. Stained cells vastly populating the EpCAM positive enumeration gate.

Supplemental Figure 1 B. Thioflavin Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the Thioflavin specific PE Fluorochrome (407nm laser, 530/30-A emission filter) (x axis; y axis = Side Scatter Profile). The corresponding unstained cytometry enumeration gate displayed no Thioflavin positive populations. Bottom Row: Histogram showing Thioflavin Antibody stained BT474 cells with high fluorescent intensity in the Pacific Orange Fluorochrome channel. Stained cells clearly displaying right shift when compared to Unstained cells.

Supplemental Figure 1 C. CD45 Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the CD45 specific PE Fluorochrome (407nm laser, 530/30-A emission filter) (x axis; y axis = Side Scatter Profile). The corresponding unstained cytometry enumeration gate displayed no CD45 positive populations. Bottom Row: Histogram showing CD45 Antibody stained BT474 cells with low fluorescent intensity in the PECy7 Fluorochrome channel. Stained cells clearly displaying no apparent shift when compared to unstained cells, thus demonstrating no expression of leukocyte-specific CD45 Antibody on BT474 cells.