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Team Cherry Picker Blake Hondl Amit Mehta Jon Millin Ryan Pope

Team Small Molecule Blake Hondl Amit Mehta Jon Millin Ryan Pope

Client & Advisor Noël R. Peters, M.S. Keck-UWCCC Small Molecule Screening Facility Professor Willis Tompkins Department of Biomedical Engineering

Abstract High throughput screening (HTS) involves secondary testing of chemicals that initially show desired characteristics High throughput screening (HTS) involves secondary testing of chemicals that initially show desired characteristics –Done manually and prone to errors Goal is to design a device to guide a micropipette user to a single microtiter well Goal is to design a device to guide a micropipette user to a single microtiter well The device will increase efficiency and reduce user error The device will increase efficiency and reduce user error Device implementations Device implementations –Java Application –LCD Screen –User Interface

Problem Statement Design a device to guide a micropipette user in the transfer of small volumes of compounds between 384-well microtiter plates Design a device to guide a micropipette user in the transfer of small volumes of compounds between 384-well microtiter plates The device will increase the efficiency of transfers and reduce user error The device will increase the efficiency of transfers and reduce user error

Background High Throughput Screening High Throughput Screening –Used in cancer and RNA research –Secondary testing performed on the chemicals that initially show promise –Use of plate reader to determine potential “hits” EnVison® Plate Reader

Motivation Secondary testing is typically done manually, making it difficult and prone to errors Secondary testing is typically done manually, making it difficult and prone to errors Row and column identification is necessary Row and column identification is necessary –Interior wells are difficult to locate Errors lead to increased time costs Errors lead to increased time costs –Only step in the process that is prone to significant human error

Current Competition Matrix Memowell ® Matrix Memowell ® –96 well pipetting aid –Lights up 4 wells when used with 384-well plate –Costs approx. $1000 Quadra Cherry Picker Quadra Cherry Picker –Automated –Costs $150,

Design Constraints Must interface with Microsoft  Excel  and user Must interface with Microsoft  Excel  and user Effectively guide the user to the appropriate wells Effectively guide the user to the appropriate wells Minimize required bench space Minimize required bench space –Dimensions smaller than 8.5” x 11” Withstand exposure to various chemicals and reagents Withstand exposure to various chemicals and reagents –Dimethyl Sulfoxide Lightweight (<10 lbs) and inexpensive (<$1000) Lightweight (<10 lbs) and inexpensive (<$1000)

Final Design Java Application Java Application –User input to locate wells in one or two plates –Excel input from spreadsheet (future work) LCD Screen to show output from Java Application LCD Screen to show output from Java Application –Lines (cross-hairs) displayed to identify wells to user

Final Design Protection of the LCD screen Protection of the LCD screen –Hard acrylic sheet protects LCD screen from pressure and possesses good optical clarity –Polypropylene sheet to protect from the Dimethyl Sulfoxide buffer Maintaining the position of well plates on LCD screen Maintaining the position of well plates on LCD screen –Two sets of tracks guide the movement of “arms” which will fix the plates in place

Prototype Construction Purchased refurbished LCD screen Purchased refurbished LCD screen –10.4” Screen –Inverter –Ribbon cable Needs to be refreshed at 60 Hz Needs to be refreshed at 60 Hz –Too fast for serial or USB port

Prototype Construction Acquired materials Acquired materials –Laptop: Samsung SENS 800 –Aluminum sheet metal –Polypropylene cover –Non-glare 1/8” acrylic sheet Detached screen from base Detached screen from base –Purchased components to extend screen  30 connector HD ribbon cable

Programming User interface User interface –User may choose between:  User input or Microsoft  Excel  file input  Using one well plate or two Full screen display Full screen display  Displays rectangle for definition of well plate boundaries  Displays intersecting lines according to user input, illuminating a single well Well plate on LCD screen

Design Analysis Advantages Advantages –Ease of use –Manufacturing –Adaptability –Cost –Marketability –Reduces errors –Increases productivity Disadvantages Disadvantages –Requires computer interface –Requires purchasing of hardware as well as software. –Software upgrades to enable adaptability

Cost Analysis Used Laptop from SWAP =$ Used Laptop from SWAP =$ /16” Aluminum Sheet (8 ft 2 ) =$ /16” Aluminum Sheet (8 ft 2 ) =$33.36 Acrylic Sheet (2) =$ 3.90 Acrylic Sheet (2) =$ 3.90 Polypropylene cover =$ 0.20 Polypropylene cover =$ 0.20 Flat Ribbon Cable & Connector =$16.71 Flat Ribbon Cable & Connector =$16.71 Miscellaneous Components =$ 7.00 Miscellaneous Components =$ 7.00 Total=$ Total=$ Cost to purchase a new LCD flat screen for a computer $300 (Future Work) Cost to purchase a new LCD flat screen for a computer $300 (Future Work)

Future Work Implement additional programming code Implement additional programming code –Allow the program to take input from an Excel  file Conduct initial testing on laptop prototype Conduct initial testing on laptop prototype Create interface between prototype and PC Create interface between prototype and PC Design an optimal structure for the prototype, emphasizing ease of use for the user Design an optimal structure for the prototype, emphasizing ease of use for the user Confirm prototype’s resistance to chemical solvents encountered in the lab Confirm prototype’s resistance to chemical solvents encountered in the lab

References “Digikey.” Digi-key Corporation. Retrieved on April 15, “Digikey.” Digi-key Corporation. Retrieved on April 15, “Java Excel API Tutorial”. “Java Excel API Tutorial”. “LCD Basics: Monitor Technology 101.” ViewSonic. “LCD Basics: Monitor Technology 101.” ViewSonic. “LCD Video Controller.” Subassembly Product Guide. “LCD Video Controller.” Subassembly Product Guide. “Matrix Memowell.” Matrix Technologies. Retrieved on January 23, “Matrix Memowell.” Matrix Technologies. Retrieved on January 23, Peters, Noël. Personal Interview. January 30, Peters, Noël. Personal Interview. January 30, “Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept. LG. Philips LCD Co., Ltd. A1_CAS(Ver0.1).pdf. “Product Specification for LB104V03 Liquid Crystal Display.” Products Engineering Dept. LG. Philips LCD Co., Ltd. A1_CAS(Ver0.1).pdf. “Quadra Cherry Picker.” Tomtec. Retrieved on January 29, “Quadra Cherry Picker.” Tomtec. Retrieved on January 29, “Samsung.” Samsung Electronics. Retrieved on April 22, “Samsung.” Samsung Electronics. Retrieved on April 22, Schowalter, Jeff. Personal Interview. March 26, Schowalter, Jeff. Personal Interview. March 26, “Small Molecule Screening Facility.” UW-CCC. Retrieved on January 23, “Small Molecule Screening Facility.” UW-CCC. Retrieved on January 23, Wu, Thomas C. “An Introduction to Object-Oriented Programming with Java (Third Edition)” and imported classes from JavaBook. Wu, Thomas C. “An Introduction to Object-Oriented Programming with Java (Third Edition)” and imported classes from JavaBook.