Lox Site Random Non-coding DNA (1-2kb) Stop Codons Antibiotic Resist Cre Pops Blocker This device will prevent Pops from a promoter reaching target genes.

Slides:

Advertisements

Similar presentations

Jeopardy Genetic Engineering Bones and Muscles Misc.Nervous System Q $100 Q $200 Q $300 Q $400 Q $500 Q $100 Q $200 Q $300 Q $400 Q $500 Final Jeopardy.

Advertisements

5 Stages involved in GE Isolation Cutting Ligation and Insertion

Control of Expression In Bacteria –Part 1

Genetic Jigsaw Class instructions. Start of lesson Divide the classes into 6 groups: – Origin of replication – Repressor gene – Promoter – Multiple cloning.

Control of Microbial Growth Tim Ho University of Alberta, Canada * The materials are mostly based on Dr. Brian Lanoil’s Microb Part.

Chapter 4: recombinant DNA

Recombinant DNA Technology

SBI 4U November 14 th, What is the central dogma? 2. Where does translation occur in the cell? 3. Where does transcription occur in the cell?

Mutagenesis Methods Lily Peterson April 5 th, 2010.

Transfection The students need to have some background knowledge about recombinant DNA technology for this lecture. Key words: Transient transfection,

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method.

Genetic Jigsaw Class instructions. Start of lesson Divide the classes into 6 groups: – Origin of replication – Repressor gene – Promoter – Multiple cloning.

RECOMBINANT DNA TECHNIQUE

Gene regulation  Two types of genes: 1)Structural genes – encode specific proteins 2)Regulatory genes – control the level of activity of structural genes.

Biotechnology Packet #26 Chapter #9. Introduction Since the 1970’s, humans have been attempted to manipulate and modify genes in a way that was somewhat.

Genetic transformation of E. coli bacteria

Do Now 3/20 OBJECTIVES: Define plasmid and restriction enzyme. Identify where a restriction enzyme will cut a DNA sequence. Explain how REs and plasmids.

Protein Synthesis Also Known As … Decoding the Central Dogma.

Transcription Transcription is the synthesis of mRNA from a section of DNA. Transcription of a gene starts from a region of DNA known as the promoter.

Lecture 7 Microbial Genetics: Genetic Mutations Gene Transfer.

Cloning and rDNA (II) Dr. Abdulaziz Almalik

Yesterday: Genetic Disorders and Gene Therapy

Biotechnology Packet #12 Chapter #9. Introduction Since the 1970’s, humans have been attempted to manipulate and modify genes in a way that was somewhat.

Location of features Human cytomegalovirus (CMV) immediate early promoter: 1–589 Enhancer region: 59–465; TATA box: 554–560; Transcription start point:

Basic DNA Science BE Bootcamp 2008 Phillips Group / Caltech.

Bacterial Genetics Supplemental instruction Designed by Pyeongsug Kim ©2010 Fall 2010 For Dr. Wright’s Bio 7/27 Class Picture.

Biotech Lab #1 -Extraction Extract DNA from an organism’s cell to get the GOI – gene of interest.

Cloning Genes Gene cloning: amplifying a specific piece of DNA via a bacteria cell Cloning vector: a replicating DNA molecule attached with a foreign DNA.

GenePolypeptide Gene  Polypeptide Transcription 1.RNAP binds to promoter 2.Separates DNA strands 3.Transcribes the DNA (adds RNA nucleotides in a 5'-3'

Recombinant DNA What is the basis of recombinant DNA technology? How does one “clone” a gene? How are genetically modified organisms (GMOs) created? Illustration.

Bioengineering Turning Genes on and off in Bacteria.

SEQUENCING DNA Jos. J. Schall Biology Department University of Vermont.

Bacterial Plasmids Loops of DNA found in some bacteria

Protein Synthesis- Transcription DNA-->RNA. Expression of Gene or Protein Synthesis I. Transcription A. Initiation B. Elongation C. Termination D. RNA.

Cloning the omcF gene from geobacter sulferreducens to E. coli

1 Coding region ATG: Translation start Translation stop Transcription start (mRNA start) (mRNA end) poly-adenylation exon intron Mature mRNA Gene structure.

Cell Transformation Recombinant DNA Host Cell DNA Target gene Modified Host Cell DNA.

GENETIC TRANSFORMATION. WHAT IS GENETIC TRANSFORMATION Genetic transformation is altering a cell by adding genetic information from an outside source.

CFE Higher Biology DNA and the Genome Transcription.

AIM: Genetic Engineering: changing the DNA of living organisms. 1. Inserting genes into other organisms 2. Selective Breeding 3. Cloning.

Cloning Vectors Enable DNA molecules to be replicated inside host (e.g., bacteria) cells. Features: 1. Origin of replication (ORI) 2. Cloning sites =

Aim #68: What are some applications of Genetic Engineering? Genetic Engineering is a process that is used to the alter the genetic instructions in organisms.

Gene Expression Chapter 16. DNA regulatory sequence All on DNA Promoters – Start transcription Promoters – Start transcription Terminators – End Transcription.

DNA Isolation. Nucleic Acid Structure & Function DNA & RNA are composed of Nucleotides A nucleotide consists of three covalently-linked parts: –A nitrogen.

Gene of interest 203 base pairs surrounds and promotes transcription of many ( 85%) of GMO genes 225 base pairs Stops the transcription of most GMO genes.

Topics to be covers Basic features present on plasmids

Genetically Engineered Mouse Models of Prostate Cancer

Using Plasmids in Biotechnology

Aim: What are some applications of Genetic Engineering?

Accelerated Biology Transformation Lab

13-3 Cell Transformation Interactive pgs. 329.

Ch. 13 Genetic Engineering

AHL 7.2 AHL 7.2 AHL 7.2

Step 1: amplification and cloning procedures

Gene Expression 1. Gene expression is the activation of a gene that results in transcription and the production of mRNA. Only a fraction of any cell’s.

Cloning the omcF gene from geobacter sulferreducens to E. coli

Accelerated Biology Transformation Lab

Volume 74, Issue 4, Pages (August 2008)

Rationale for generation of reporter fusions by Red-mediated recombination. Rationale for generation of reporter fusions by Red-mediated recombination.

Chapter 9 Molecular Genetic Techniques and Genomics

Functional Analysis of Genes

Genetically Engineered Mouse Models of Prostate Cancer

Aim: What are some applications of Genetic Engineering?

Transcription Protein Synthesis.

Bacterial Transformation

Figure 1. Kidney-Specific Cre/loxP Recombination.

B7 Variation and Evolution- Paper2 Revision

FOR Stage 1 Stage 2 Stage 3 J37015 J37016 J37019 J37020 J37025A

Presentation transcript:

Lox Site Random Non-coding DNA (1-2kb) Stop Codons Antibiotic Resist Cre Pops Blocker This device will prevent Pops from a promoter reaching target genes. Activation of the Cre Recombinase genes will remove the stop codons and allow Pops to pass through this part. Removal of the stop codons also allows transcription of an antibiotic so the activated bacteria can be selected. The restriction sites allow the part to be ligated into a system in the normal fashion. This part can only be activated once and will remain activated forever. It should be 100% efficiant at stopping Pops passing through it until activated. No other molecules are required for any of the steps, simple. A separate induceable Cre plasmid must be inserted to produce the cre recombinase Antibiotic Resist

How Cre Works Cre is under the control of a promoter, there are commercially available Cre containing plasmids

How to make this in 2 hrs How to make this in 2 hrs Lox Site Stop Codons Random DNA Antibiotic Resistance Restriction sites Primer 1 Primer 2 Primer 3 (Low Concentration) Random DNAAntibiotic Resistance John Chattaway and Jonny Wells