Super-resolution-nanoscopy Resolvable volumes obtained with current commercial super-resolution microscopes. Schermelleh L et al. JCB 2010;190:165-175.

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Presentation transcript:

Super-resolution-nanoscopy Resolvable volumes obtained with current commercial super-resolution microscopes. Schermelleh L et al. JCB 2010;190:

Super-resolution imaging principles. Schermelleh L et al. JCB 2010;190:

Super-resolution microscopy of biological samples. Schermelleh L et al. JCB 2010;190:

Several techniques that produce images with resolution better than the confocal microscope. Lidke D S, and Lidke K A J Cell Sci 2012;125: PI—2 two opposing lenses ~100-nm axial resolution (right) reveals mitochondrial fragmentation that was not apparent in confocal microscopy images (center) SIM- series of images are collected while scanning the illumination pattern across the sample 3D images of nuclear pore complex epitopes (red) nuclear lamina (green). DNA(blue) Resolution 100 by 300nm ‘Bessel’ beam swept through the sample 300-nm isotropic resolution combined faster imaging dramatically reduced photo-bleaching confocal microscopy (top center) and Bessel beam imaging (bottom center). Single 3D frame(right) bar=10 μm. 3D STED with 50 by100-nm resolution. Images of neurofilaments. Confocal (center) & 3D STED (right).

Sub-diffraction imaging through photobleaching microscopy Works for data collected from confocal or wide-field microscope