MICROSCOPES Unit 1 – Intro to Science
Types of Microscopes
Electron Microscope Uses a beam of electrons to magnify image greater than 1000x (1000 times) Kills observable cells so not used to view live specimens.
Compound Light Light passes through lenses to magnify image up to 1000X Used to observe living specimen. Most commonly used microscope in biology Compound because of multiple objectives.
Dissecting Microscope Used for low magnification of three- dimensional objects- objects larger or thicker than the compound microscope can accommodate. Magnifies between 10 and 40 times (10x- 40x)
Eyepiece Body Tube Revolving Nosepiece Arm Objective Lens Stage Stage Clips Coarse Focus Fine Focus Base Diaphragm Light Microscope Parts
Microscope Magnification To calculate the power of total magnification, multiply the power of the ocular lens by the power of the objective. On our compound microscopes we have a 4, 10 and 40 objective. The ocular lens (eye piece) magnifies 10 times. Total magnification when looking in the microscope on the 4 power objective would be what? ________
Microscope Facts When focusing on a specimen always start by using the scanning objective. When using high power use only the fine adjustment knob. The microscope will flip and invert an image The higher the objective the less of an object you can see (pay attention to these things when looking at the “e” When in doubt, ask your teacher!!
Microscope care Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords