Lab Notebook Format 1) Title/Date 2) Pre-lab (Parts A, B, C) 3) Purposes (2-3) 4) Personal Account 5) Discussion– only 1 question (on bottom of pg 3 of.

Slides:

Advertisements

Similar presentations

Enzyme Activity Lab 13 AP Biology

Advertisements

Use of the Spectronic 20 Spectrophotometer

Convection Density of Fluids Investigation 5, Part 1.

Investigation 1 What is the relationship between the concentration of a solution and the amount of transmitted light through the solution?

Module 3 - Photosynthesis

Spectrophotometers and Concentration Assays

Hemoglobin concentration

Colorimetry & Spectrophotometry.

Hemoglobin Experiment

Equilibrium Constant Turn in CV Final Report and Pre-lab in folders

Lab 6: Enzyme Catalysis.

Enzyme Catalysis with Catalase

Word of the Day Denature - changes in the chemical structure of an enzyme.

Introduction to Spectrophotometry. Why Spectrophotometry? Imagine you are to make a 1μM solution of a specific protein that you believe could have anti-carcinogenic.

Making Solutions of Differing Mass/Volume Concentrations

Wednesday, 9/12/12 Finish Toothpickase Activity  must be completed today!!! – Due tomorrow!!!!! 1 st lab this week – set up today, lab Thursday & Friday.

Enzyme Catalysis with Catalase

Edvotek kit # 282. Why? For Biology II or AP biology Follow up to:Introduction to  Protein structure & function  Properties of enzymes  Factors that.

CHEM 213 Instrumental Analysis

CHEM 251 Laboratory Chemical Kinetics Lab. Agenda For the week of November 29 th Experiment: Iodine Clock, handout Prelab Quiz: Material in the handout.

Chemical Kinetics TURN ON SPECs NOW!. First things first… Safety: Put bags away Goggles Lab Jacket Gloves Basic Reactants! LAB! Warm up Spec 20.

Enzyme Activity Lab 13 AP Biology

Scientific Method Unit 2. We will set up the scientific method experiment today and will work on it for the next 2 weeks. Mealworm or Tenebrio molitor.

Lab 1: Kinetic Rate Law of CV+OH - Turn in your prelab OPEN Last Week’s Spreadsheet(s) Today: Study the Kinetic Rate Law of Reaction: Crystal Violet (C.

Rate Law and Activation Energy Methyl Blue

AP Photosynthesis Lab

Determination of the Rate of the Light Dependent Pathway as measured by the reduction of DPIP PHOTOSYNTHESIS LAB.

Dilution and Spectroscopy Lab Lab Word Document Lab Word Document.

Colorimetry & Spectrophotometry

Visible Spectrophotometer

SPECTROPHOTOMETRY IN BIOTECHNOLOGY

(glass pipette, centrifuge, spectrophotometry)

Electromagnetic radiation travels through space in the form of a wave, with the distance between two crests of a wave termed a wavelength. At one end.

Unit 10 Photosynthesis Plants use energy from the sun and convert it to chemical energy –Glucose Photosynthesis occurs in chloroplasts Capture of light.

New Lab Report Format Chemistry II.

Exp. 18: Spectrophotometric Analysis: Concentration of a Solution Using Beer’s Law White light violet (400nm) – red (800nm) “visible spectrum” sample (light.

SPECTROSCOPY. Kendall Olds Derrick Woods Ricketta Johnson Carl Flowers.

An Introduction to the Spectrophotometer. Meet your Spectrophotometer Meet your spectrophotometer.

Student Directed Investigation – due dates. Due Dates February 13 – Methods & Materials Section due (in Google drive – shared with me) February 13 – Introduction.

Peroxidase lab. What is peroxidase?  All organisms have enzymes (catalysts) called peroxidases that break down hydrogen peroxide into less harmful substance.

Spectrophotometry Ability of molecules to absorb and transmit light energy is the basis of one of the most widely used procedures for determining the concentration.

Agenda Collect Pre-lab #6 Lab Quiz #5 Paper Assignment due

Experiment 33 Colorimetric Determination of Iron CHE1181.

Photosynthesis Lab - Elodea & Bromothymol Blue

Absorption Spectroscopy CHEM 251 Week of November 1 st, 2010 Alexis Patanarut.

LAB FOUR Photosynthesis. 4A Plant Pigment Chromatography 4B Tracking the Light Reaction LAB FOUR Photosynthesis.

Topic 1 Tools of the Biology Laboratory Exercise I. The spectrophotometer. Exercise II. Liquid Measurements. Exercise III. Sedimentation and Centrifugation.

Lab Notebook Setup. Title Page Fill out in permanent ink AP Biology Lab Notebook Name Period Mrs. Perry, Room

Experiment: Solutions Preparation, Part 1 1CHE116.

Absorption Spectrum for Plant Pigments

Spectrophotometry Measuring Concentrations of Substances in Body Fluids.

How much protein is really in my… -milk? -soy “milk”?

Lab1 A VISIBLE ABSORPTION SPECTROMETER

SPECTROPHOTOMETRY White light contains all wavelengths of light in the visible region of the EM. A substance appears colored because it absorbs light.

Solutions & Spectrophotometry

Colorimetric Determination of Iron

Spectrophotometric Determination [Co2+]

SPECTROPHOTOMETRY White light contains all wavelengths of light in the visible region of the EM. A substance appears colored because it absorbs light.

SIDEBAR: PLANTS ABSORB A LOT OF LIGHT ENERGY…

Concentration determination of acetylsalicylic acid by using ultraviolet spectrometer Liang Zhang 03/25/2016.

Absorption Spectroscopy

Colorimetric Determination of Keq

Experiment 4-4: Determination of Glucose in Blood Serum

Density of Fluids Investigation 5, Part 1

Purpose of the Lab 3E, 3F, 3H Learn how to use a spec to figure out concentration of solutions Learn how to use a GLX Practice application of calculations.

Cu2+ + 4NH3 → Cu(NH3)42+ (deep blue)

3.1 Building a Density Column

Students Get handout - FRQs LL mid-point Monday

Solution Preparation Experiment

Students Get handout – Enzyme Lab (if absent yesterday)

Presentation transcript:

Lab Notebook Format 1) Title/Date 2) Pre-lab (Parts A, B, C) 3) Purposes (2-3) 4) Personal Account 5) Discussion– only 1 question (on bottom of pg 3 of packet) 6) Conclusion

Lab 2: Enzyme catalysis Guaiacol + 2H 2 O 2 tetraguaiacol + 8H 2 O (colorless) (brownish orange) Enzyme = peroxidase Substrate = peroxide (H 2 O 2 ) How will we measure enzyme function? - Color change using a spectrophotometer peroxidase

How does a spectrophotometer work? Take home points 1.Light at a specific wavelength is sent through a liquid sample 2.Light passing through the sample is detected on the other side 3.To measure this accurately, we must calibrate (blank) the spec. 4.Calibrating the spec sets the range & “tells” the spec: -what 0% light is (darkness) -what 100% light is (all light passing through) -For our experiment we will see how the amount of light passing through changes as the reaction proceeds.

Tube #1 (blank) Tube #2Tube #3 Guaiacol0.1 mL (2 drops) 0.1 mL (2 drops) --- H2O2H2O2 0.2 mL (4 drops) --- Turnip extract (peroxidase) 1.0 mL (20 drops) mL (20 drops) dH 2 O5.2 mL5.0 mL--- 1.Set wavelength to 500 nm 2.With the chamber empty, use left knob to adjust to 0% T 3.Insert tube #1 (blank) into spec & use right knob to adjust to 100% T 4.Pour #2 into #3 & begin timing immediately!! 5.Invert 2X quickly & place into spec 6.Record %T every 10 sec for 120 secs 7.From page 3, choose your experiment for tomorrow

Enzyme Lab Overview – Only hypothesis/procedure/data from THURSDAY needs to be included. – Slope calculation for EACH of your four lines ΔY / ΔX(Units = Y units / X units) – Discussion section (section #5) is only ONE question! Math Overview – Be sure you’re clear on how to solve the first four problems on the Unit 3 Math Practice sheet!