Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, IPK Genebank – Satellite Collections North – Oil and Fodder Crops Collection Malchow / Poel

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Sites of Research Group Malchow/Poel Groß Lüsewitz

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Gramineae (Grasses) Leguminosae (Red clover and Alfalfa) Cruciferae (Oilplants) Collections of Oil and Fodder Crops Total: Accessions, 23 Genus and 115 Species

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Tasks of Satellite Collections Collecting and Seed exchange Maintenance (Regeneration and Storage) Characterization and Evaluation Documentation Collection specific Research (Marker analysis) Service (Information and seed samples provision)

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Cultivation and Regeneration of Cruciferae and Leguminosae in cagesPlastic bags isolation in Field in isolation tents in Greenhouse Pollination with bumble bees or wild bee Osmea rufa)

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Regeneration, C&E-Cultivation of Gramineae Trials in Rye field Isolation plot in Rye field

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Freezing room for grasses (-5°C)Base collection (-20°C) Storage of active and base collections

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Storage of Safety duplicates Since 2008 Storage in the SGSV Svalbard Global Seed Vault, Spitzbergen (Norway) Aim: Protection of Genetic ressources against disaster, climate change, wars and unrests, epidemics, a.s.o. SCN:Status of storage: 8778 Oil and Fodder crops accessions, thereof: 6671 Fodder crops

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Results of PGR work Service: 2101 seed samples worldwide provided - Germination tests: 5449 accessions checked - Cultivation: 1048 acc. for regeneration, characterization and evaluation 61% of all O&F-accessions under management per year !

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Forage grasses - Poa Central Crop Database Development and assigning Most original sample towards defining an European Forage Collection (EFC) Evelin Willner: Baltic Sea Region WS, POA-EDB-MOS,

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Selection of candidates for European Forage Collections (EFC = forages MAAs) 1997:Beitostolen, Norway, objectives and scope of the European Forage Collection were defined (see also 6th Meeting - Report of WG on Forages, p ) 1999:Elvas, Portugal, a procedure to identify MOS (most original samples) was proposed (see also 7th Meeting - Report of WG on Forages, App I, p ) 2003:Linz, Austria, CCDB managers should make a proposal for Holder of primary collection (PRIMCOLL), focusing on 7 priority crops, Discussion about regeneration standards (see also 8th Meeting - Report of WG on Forages, App I, p )

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, :Lindau, Switzerland, progress was made in MOS definition of the priority crops, forage specific descriptors were revised (slide # 19) 2007:Piestany, Slovakia, progress mainly for Poa and Phleum, only partially for other crops Selection of candidates for European Forage Collections (EFC = forages MAAs)

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Definition of selection criteria 1.MOS determination: an accession is a most original sample, when his origin is known and GB is COL or BRE 2.PRIMCOLL determ.: a primary holder should be the GB in the country where the accession was collected or bred 3.Primary holder: the GB works according regeneration standards for forages (acceptable or preferred standard) 4. MOS accession: seed is free available 5.MOS accession: has a safety duplicate

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, First step: Original Status (OS) 1. Most original sample (MOS) Gene bank is collector or breeder of the material. 2. With MOS The material of a donor has been repatriated by the gene bank; the gene bank is collector or breeder. 3. One away Gene bank obtained the material from a donor which is collector or breeder of the material. 4. More away Gene bank obtained the material from a donor which is not collector or breeder of the material, but another institute or breeding company. 5. Unknown Donor of the sample is unknown.

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Originality (EURISCO code) bredcollectedUnknownTotal (1) MOS (2) with MOS (3) one away (4) more away (5) Unknown total Definition most original sample (MOS) for Poa sp.

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Second step: PRIMCOLL 1.Primary Holder/ GB in Country of Origin (MOS) 2. Primary Holder/ GB is not Country of Origin, collected material is original (MOS) Repatriation? yes no Maintenance of MOS in GB in Country of Origin Maintenance of MOS in GB which is not in Country of Origin Candidates for EFC

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Third step: PRIMARY HOLDER Maintenance in country of origin according to genebank standards Maintenance in other countries according to genebank standards Free available Safety duplicate + MOS EFC (Characterization and/ or Evaluation data)

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, PRIMARY HOLDER: GB regeneration standards Item of regeneration procedurePreferredAcceptable Site (greenhouse/cabins, field/cages, field/isolation)yes Insects as pollinator Crop used as isolation (specify; n.a. for cages) Isolation distance between plots (n.a. for cages)length >50 m; width >50 m Plants per accession (number)10030 Distance between single plants0.20 m Scoring of traits Selection in accessions (yes / no)yes Harvesting (once / several (times) as balanced / bulk unbalanced)balancedunbalanced Dryingdry roomdrying equipm. Threshing and cleaningmanualwith machines Final drying (temperature, relative humidity (specify)final moisture content)3-7% Viability testing before storage (yes/no)yes Seed packaging and storage (base and duplicate collection per plant bulk active collection balanced bulk bulk) per plant balanced bulk bulk Information management IT basedIT based (8th Meeting - Report of WG on Forages, App I, p )

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Descriptor list of Forages/ All Descriptors of EURISCO Seed availability Originality (MOS) PRIMCOLL EFC Additional descriptors + Habitat-, Collection- and Breeding-Description

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Poa sp. - Database/ Germany In total 5719 Acc. ITA363 SVN019 FRA243 LVA009 TUR001 CHE002 ROM003 BEL004 AUT060 POL022 DEU146 SWE054 CZE082 HUN003 GBR016 SVK001 BGR001 – 2% LTU001 – 3% NLD037 – 1% 48% 21% 3% 4% 8% 3% 5% 2%

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Candidates for European Forage Collections updated Poa sp. - Database 2010 Poa sp. - accessions5719 (100%) ORIGINALITY (MOS: 3991?)5629 (98%) PRIMCOLL5234 (92%) Confirmation Curator2441 (43%) Suggestion by DBmanager2793 (49%) Candidates for EFC: yes 781 no 398 (14%)

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Conclusions - Updates, close gaps in the passport data - Most important descriptors: ACCNAME, ORIGCTY, SAMPSTAT, COLLSITE, COLLNUMB, DONORCODE, DONORNUMB, SEEDAVAIL, SAFETYDUPL... - Correctness of data, Good Scientific Practice - Quality management system

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Grasses Collection Lolium perenne L. (Perennial ryegrass) 2968 accessions Festuca pratensis Huds. (Meadow fescue) 1119 accessions

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Dactylis glomerata L. (Cocksfoot) 1862 accessions Phleum pratense L. (Timothee) 983 accessions Poa pratensis L. (Smooth meadow grass) 587 accessions Grasses Collection

Evelin Willner: Baltic Sea Region WS, Poa sp.-EDB-MOS, Collection of Cruciferae, no of accessions Brassica napus... f. annua (Spring rapeseed) 236 Brassica napus... f. biennis (Winter rapeseed) 885 Brassica rapa (Oilseed turnip) 248 Brassica juncea (Brown mustard) 308 Sinapis sp. (Charlock, White mustard) 234 Brassica oleracea.... medullosa (Forage kale) 223 Brassica nigra (Black mustard) 124 Turnip Forage kaleWhite mustard Rapeseed