HOW MUCH DOES OZONE THERAPY NEED A REAL BIOCHEMICAL CONTROL SYSTEM? THE OXIDATIVE STRESS IMPORTANCE AND ASSESMENT FRANK HERNÁNDEZ Ozone Research Center. Cuba

Why ozone in medicine has not been approved as a common practice by world medical community? The use of specific parameters to control the applicable dose in terms of safety and therapeutic effects has been gaining importance Ozone Therapy

Ozone therapy and the Oxidative stress Mechanism of action Regulator of endogenous Oxidative Stress Toxic effects Therefore, the oxidative stress phenomenon is deeply involved in ozone therapy effects. A test or system for assessing oxidative stress process will be of great relevance

Ozone therapy and the Oxidative stress Oxidative stress can be defined as the prevalence withing the living cell of oxidizing activities over the cellular antioxidant defense activities. Complex Biological Process Different Oxidant substances Different Antioxidant compounds

ESPECIES REACTIVAS DEL OXIGENO Especie MolecularVida media (seg C HO · 1 x RO · 1 x ROO · 0.1 – 7 x O 2 ·- 1 x (SOD) 1O21O2 1 x NO · H2O2H2O2 2.5 (plasma) HOCLRegularmente estable O=NOO · 1 – 4

Oxidative Stress from Ozone Therapy Oxidant Substances Criege Ozonides Hydrogen Peroxides Hydroxyperoxides Aldhydes Antioxidant Compounds GPx GSH GST SOD CAT ADH

System for Oxidative Stress Diagnosis 3 mL of Blood PlasmaErythrocytes Conjugated Dienes Thyl-Barbituric Acid Reactive Substances Total Hydroperoxides Reduced Glutathione Glutathione Peroxidase Glutathione S-Transferase Superoxide Dismutase Catalase

SISTEMA DE DIAGNÓSTICO DEL ESTRÉS OXIDATIVO System for Oxidative Stress diagnosis Total Antioxidant Activity Total Pro-oxidant Activity Redox Index Grade

PARAMETERSREFERENCE Total Antioxidant Activity (Units) – Actividad Prooxidante Total (Unidades) – Indice Redox0.924 – Grado0 Oxidative Stress Diagnosis

Grades of oxidative stress and their clinical meaning Grade Meaning 0 No oxidative stress 1 Light oxidative stress 2 Moderate oxidative stress 3 Severe oxidative stress 4 Very severe oxidative stress

Oxidative Stress Diagnosis of cardiopathy Patient O3T Sessions Total Antioxidant Activity Total Pro- oxidant Activity Redox Index Grade

Using Oxidative Stress Diagnosis in Ozone therapy Grade 0: Any range of concentration and dosage. Grade 1: From top level of medium range to downward. Grade 2: From minimal level of medium range to downward. Grade 3: Minimal level of low range. Grade 4: No ozone therapy.