The microwave multifunctional KOS The microwave multifunctional tissue processor you were waiting for

In a fraction of the time KOS Tissue processing Decalcification Special stains Gross hardening Fixation Antigen retrieval (93°C to 120°C) In a fraction of the time

The first Icon driven HistoStation KOS: Smart and Fast The first Icon driven HistoStation

KOS* Multifunctional: 1 unit 6 applications Fast: Just plug-in and process Reliable: Sensors controlled Flexible: User programs / Favorites Safe: Sensors monitored Smart: Icons driven 5

Multifunctional: ONE unit SIX major applications Fulfilling all requirements set by CAP/C.L.S.I. Fulfilling all requirements set by CAP/C.L.S.I. Ideal substitute for “kitchen type” microwave oven Ideal substitute for “kitchen type” microwave oven

Rapid Tissue processing Same day results for all tissue types. From 1 to 45 cassettes Ideal for transplant center Urgent biopsies Up to 3 mm thickness Safe. No xylene Ethanol – isopropanol only 7

tissue processing time Fast, unmatched tissue processing time . 35 minutes For transplant biopsies including fixation 75 minutes For 2 mm biopsies Add 18 min fixation 140 minutes For 3 mm biopsies Add 28 min fixation

High Quality Slides Excellent H&E, IHC and FISH In a fraction of the time Colonic Biopsy H&E, x400 Adenocarcinoma Cytokaratin CAM 5.2, x400 Breast Red-Labeled HER2-FISH, x800 Breast, HER2 IHC, x400

SMART . ICONS DRIVEN MENU 2) Select number of cassettes 1) Select thickness . 2) Select number of cassettes .

3) Select reagents 4) Press GO

5) Press START. That’s all Time left will be shown on the terminal

Bone Marrow processing Same day diagnosis for bone marrow trephine biopsies. Decalcification + processing in less than 3 hours EDTA. Decalcification: 80 minutes Processing: 60 minutes Total time: 140 minutes FORMIC ACID (10%). Decalcification: 20 minutes Processing: 60 minutes Total time: 80 minutes 13

Rapid Bone decalcification Up to 45 cassette – 60 Mega cassette Hours, not days Magnetic stirring plus microwave energy Positively influence the equilibrium 1. Select temperature, 2. Number of cassette, 3. Decalcifyng solution Press GO 14

Up to 76% time saving for staining protocols Special Stains Up to 16 slides Up to 76% time saving for staining protocols 16

2) Select specific stain 1) Select stains menu 2) Select specific stain

3) Follow step by step indications 4) Selected program is prestored ready to use.

Time At Temperature Protocols Antigen Retrieval Time At Temperature Protocols Up to 80 slides 20

Dedicated Histomodules Decal, up to 60 Megacassette 4K, up to 4 cassette 45K, up to 45 cassette 16s, up to 16 slides 40s, up to 80 slides

Fixation Dedicated fixation histomodule up to 45 cassette Select time at temperature protocol to fulfill all requirements of standardization and documentation for CAP/ASCO for HER2/ER. Factory stored opitimized protocols at 37°C - 50°C guarantee consistent results 22

Gross Hardening Dedicated GHK hardening module Efficient & rapid hardening in physiologic solution for a timely grossing Factory stored opitimized protocols at 50°C – 65°C guarantee consistent results 23

Glass Pressure Reactor-120°C GPR-120°C SuperHeating Antigen Retrieval (40 slides)

Dedicated Histomodules Processing & Infiltration Resin polymerization

KOS EM demo

KOS EM demo Better Quality and TAT (2-3 days vs same day diagnosis) Users were satisfied because fully supported during demo, installation and training (Competitors were won also because of this) KOS is not automatic but users understand that gives benefits in speed + quality and price

KOS Packed with the latest microwave and sensor technology. Controlled by the most advanced, intuitive software.

Microwave is switched OFF if: State of the Art Microwave is switched OFF if: Cavity ventilation is compromised Solvent in cavity is above Threshold Humidity in cavity exceeds set value Microwave leak is detected

Any protocol as Favorite

In 3 “stainful” colors to fit the atmosphere of your laboratory Last but not least… In 3 “stainful” colors to fit the atmosphere of your laboratory Congo Red - GMS Green - Alcian Blue 33

KOS: 10 Good Reasons to Choose 1. Not a modified kitchen appliance Industrial engineered, laboratory grade, microwave unit designed specifically for histology applications. 2. Easy-to-use Why make things complicated? Our icons driven user interface allows you to access information quickly. Basic user maintenance. 3. Optimized protocols Factory stored for tissue processing, decalcification, fixation, gross hardening, special stains and EM User’s defined protocols can be stored and, if required, included in a “Favorites” menu for easy retrieval. 4. Fully compliant We have always been concerned with Safety and Quality. Our laboratory grade microwaves fully comply with CAP-C.L.S.I. Guidelines.

5. Full Tech support With over 70 years of combined histology experience our applications support professionals have the answer to your questions. 6. Specimen safety • Focused infrared automatic temperature control with laser positioning. • Software controlled magnetic stirring for homogeneous temperature distribution • Built-in re-generable charcoal filter on exhaust outlet 7. Lab safety Sensors controlled operation. Built-in sensors will switch off microwave if: • Cavity ventilation is compromised • Solvent & Humidity in cavity is above threshold • Microwave leak is detected 8. Full documentation Through a USB port, data from the built-in Logbook can be easily downloaded for documentation Purposes (Q.A. - ISO 9001 - G.L.P.) 9. TV 7’ monitoring for easy observation of process in cavity. 10. Unique histomodule technology for easy, safe handling of solutions while preserving the pH of the solution throughout the procedure.

Dual multifunctional tissue processor In a fraction of the time Milestone Histos Bench top microwave Dual multifunctional tissue processor Fixation and Tissue processing Decalcification Special stains Gross hardening In a fraction of the time

HISTOS line Processing performance Reagent efficiency Environmental sensitivity High quality results 38

Histos Processing Time Thickness 35 min (+10 min fix.) 1.15 hr (+20 min fix.) 4.30 hr (+60 min fix.) 39

Simplicity NO need to change your grossing procedure NO need for special grossing tools Software driven procedures No cleaning cycles 40

Safety Fixation with either formalin or Milestone’s FineFIX XYLENE free processing Exhaust fan Safety micro-switches Fume sensors Methods and process reports can be stored and exported 41

High throughput Histoprocessing of batches with up to 110 standard cassettes… 42

up to 16 Mega and 8 SuperMega cassettes. High throughput …and of batches with up to 16 Mega and 8 SuperMega cassettes. 43

Is in the microscopic comparison Quality equal or better than conventional histoprocessing for H&E, IHC, FISH The Proof ? Is in the microscopic comparison Excellent results in a fraction of the time No compromise in regard to morphology and “staining” expression

Benefits Vs Conventional systems Fast processing and Same day results Flexibility in the processing Results equal or better than conventional Flexibility in workflow (no peaks) Dual processing and High throughput (110 Cass in 40-240 min) More healthy environment Cost saving (reagents and wax disposal) 45

Benefits for all! Patient: - Same day results, less stress time waiting for results Histologist: - Improved working conditions - Work load with less peaks Pathologist: - “Same day” and high quality slides even for difficult tissues Administrator: - Reduced cost / cassette - Rapid turn around time 46

PRESENTING ’11 HistosLINE 47

HISTOS3 Microwave unit + single external retort (Wax module) Parallel processing, double productivity Up to 3mm thickness & 110 cassette No Vacuum Multifunctional 48

HISTOS3 small to medium size biopsies Ideal for Transplant center The HISTOS3 is designed for small to medium size histology labs small to medium size biopsies Ideal for Transplant center 49

HISTOS5 Microwave unit + double external retort (Dry module, Wax module) Parallel processing, double productivity Vacuum, 110 cassette Up to 7mm thickness Multifunctional 50

HISTOS5 is designed for hospital laboratories high throughput processor for same day routine wide variety of tissues without compromising tissue block thickness. Multifunctional unit for Pathos users 51

multiple functions concept MILESTONE concept MILESTONE multiple functions concept FLEXIBILITY Epitope Retrieval – Decalcification Fixation – Special Staining - Gross Hardening …in a single unit! 20 52

Histos: direct access 53

Histos: Double processing 54

Tissue processing Processing modules up to 110 cassette Fast protocol for urgent transplant biopsies (40’) Processing of samples up to 7mm thickness No xylene No proprietary reagents Excellent morphology, staining & IHC properties 55

Decalcification Processing modules for 110 cassette Decal + processing in as little as 80minutes No proprietary reagents – Preset protocols Decalcification with either EDTA or acids 56

Special Stains Processing modules for up to 12 slides Save up to 76% of the time vs standard method Factory optimized stored methods guarantee consistent results No proprietary reagents No splashing, constant pH and temperature 57

Epitope Retrieval Processing modules for up to 120 slides Factory opitimized stored methods guarantee consistent results Standard methods at 98°C GPR up to 120°C No splashing, constant pH and temperature 58

Gross Hardening Whole organ hardening module Physiologic solution is the reagent Efficient & rapid hardening for a timely grossing Factory stored opitimized methods guarantee consistent results 59