ng Spec2go™ quick and easy spectroscopy A portable and affordable sample quantitation device. Precise measurements of concentrations up to 1μg/μl. object shown to scale

Spec2go ™ ng ng sample 1 microliter of sample is added to each surface Closed/Sample Measurement Mode Open/Sample Loading Mode

Measurement Mode occurs at a single separation distance ng

Concentration easily calculated from absorbance measurement Absorbance Sample Concentration All based on the linear relationship between absorbance (what the Spec2go™ physically measures) and concentration (what you want to know).

At high concentrations, this linearity breaks down Absorbance Sample Concentration Caused by: strong electrostatic interactions between molecules changes in refractive index shifts in chemical equilibrium Higher concentrations require a shorter pathlength for measurement

No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising: [A] first and second anvil surfaces -at least one being moveable relative the other to any one of three positions; [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface; [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces; [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement.

No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising: [A] first and second anvil surfaces -at least one being moveable relative the other to any one of three positions; [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface; [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces; [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. The Spec2go™ only has two positions. Loading position Measuring position

No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising: [A] first and second anvil surfaces -at least one being moveable relative the other to any one of three positions; [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface; [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces; [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. Both surfaces of Spec2go™ are wet at loading position, not in the compression position. The liquid drop on the first surface never wets the opposing surface.

No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising: [A] first and second anvil surfaces -at least one being moveable relative the other to any one of three positions; [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface; [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces; [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. Samples in the Spec2go™ are not pulled from a compressed state to a measuring state. Loading position Measuring position

No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising: [A] first and second anvil surfaces -at least one being moveable relative the other to any one of three positions; [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface; [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces; [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. pin fixed plunger The measurement spacing of Spec2go™ is set by a pin and a non-adjustable plunger. Side view of Spec2go ™

No Infringement Under DOE DOE elementNanoDropSpec2go™ FunctionNanoDrop can be placed in three positions to allow for sample loading, compression, and measuring Device can be placed in two positions for sample loading and measuring WayMovable surfaces connected by a hinge with three different positions for loading, compression, and measuring Movable surfaces connected by a hinge with two different positions for loading and measuring ResultSmaller sample volumes can be analyzed Larger sample volumes than those in the NanoDrop can be analyzed Claim Element: Three positions

No Infringement Under DOE DOE elementNanoDropSpec2go™ FunctionBoth surfaces are wet WaySurfaces are brought in close enough proximity that a single drop wets both surfaces Surfaces are brought in close enough proximity that two droplets that have already wet each respective surface contact each other ResultSmall sample volumes that would only form a column of liquid in the compression position can be analyzed in the measuring position Samples must be large enough to spontaneously form a column of liquid in the measuring position Claim Element: Sample compression position … the liquid [drop] wets … both surfaces

No Infringement Under DOE DOE elementNanoDropSpec2go™ FunctionSample forms a column of liquid between optical source and detector WaySurfaces further apart compared to the compression position when liquid column is formed Surfaces do not move after liquid column is formed ResultSmall sample volumes that would only form a column of liquid in the compression position can be analyzed in the measuring position Samples must be large enough to spontaneously form a column of liquid in the measuring position Claim Element: Sample measuring position … pull the sample into a column

No Infringement Under DOE DOE elementNanoDropSpec2go™ FunctionSamples can be analyzed at multiple optical pathlengths during a measurement Samples cannot be analyzed at multiple optical pathlengths during measurement WayA computer controlled solenoid automatically adjusts the spacing between the optical source and detector during a measurement Nothing can be adjusted during measurement ResultOptical pathlength can be adjusted without losing sample Sample lost if optical pathlength is changed Claim Element: Adjustable measuring position