Planning rice breeding programs for impact Pedigree Selection in the IRRI Irrigated Breeding Program Parminder S. Virk PBGB Division
IRRI: Planning Breeding Programs for Impact Can anyone define “pedigree”?
IRRI: Planning Breeding Programs for Impact Describe the pedigree method Describe naming of pedigree at IRRI Describe crosses at IRRI Describe types of crosses Demonstrate IRRI nurseries Learning objectives
IRRI: Planning Breeding Programs for Impact The pedigree method owes its name to the pedigree record of selected plants maintained to trace ancestral relationship among such plants A detailed description of present day pedigree method was outlined by Love (1927) Pedigree Method
IRRI: Planning Breeding Programs for Impact In this method individual plants are selected starting with F 2 and in the subsequent segregating generations their progenies are tested till the progenies become homozygous. During entire operation a record of all parent-offspring relationship is kept: PEDIGREE RECORD In this method each progeny in every generation can be traced back to the original F 2 plant Pedigree describes parents, grandparents, great-grandparents,... Pedigree Method
IRRI: Planning Breeding Programs for Impact Based on cross number and serial numbers of selected plants IR numbers are assigned consecutively to the crosses (now IR80389) made at IRRI just before the hybrid seeds are incubated for germination When plant selections are made from F 2 and subsequent generations of an IR cross, numerical designations are given for each breeding line How is the naming of pedigree at IRRI?
IRRI: Planning Breeding Programs for Impact Selections from the F 2 were grown in the pedigree nursery as F 3 rows: IR2061-1, IR and so on up to IR From selection 214, three plants were grown as F 4 selections: IR , IR and IR From selection IR , 10 plants were again selected as F 5 rows These selections were designated as IR up to From selection IR , three plant selections were made and planted in the pedigree nursery as F 6 rows IR , IR and IR At maturity selection IR appeared uniform and was bulk harvested and evaluated in replicated trials Example: IR (IR28)
IRRI: Planning Breeding Programs for Impact How is the naming of pedigree in your country/institute?
IRRI: Planning Breeding Programs for Impact Procedure What are the breeding objectives? High Yield Potential Short Growth Duration Superior Grain Quality Multiple disease and insect resistance Choice of Parents?
IRRI: Planning Breeding Programs for Impact Staggered sown 3 times each season at 2-weeks interval IRRI Varieties are donors in various programs as Irrigated Rainfed Flood prone and Hybrid rice Donors for various traits (Hybridization Block)
IRRI: Planning Breeding Programs for Impact Hybridization Block
IRRI: Planning Breeding Programs for Impact Hybridization Block
IRRI: Planning Breeding Programs for Impact Field Book
IRRI: Planning Breeding Programs for Impact Summary of HBs Electronic Field Book
IRRI: Planning Breeding Programs for Impact Selected Donors
IRRI: Planning Breeding Programs for Impact Planning Crosses!
IRRI: Planning Breeding Programs for Impact For Yield, grain quality and incorporation of resistance to diseases and insects Released varieties x Elite Indica Lines Elite Indica Lines x Elite Indica Lines Elite Indica Lines x Basmati Type Lines Further improvement in Indica germplasm
IRRI: Planning Breeding Programs for Impact Basmati x Elite Indica Lines Basmati x Basmati Type Derivatives Basmati Type Derivatives x Basmati Type Derivatives Developing Basmati Type Germplasm
IRRI: Planning Breeding Programs for Impact Tropical Japonica x Tropical Japonica crosses Tropical Japonica x Korean Varieties Improving Tropical Japonicas for temperate regions (Korea)
IRRI: Planning Breeding Programs for Impact For Yield, grain quality and incorporation of resistance to diseases and insects Improved NPTs x Improved NPTs Improved NPTs x Released Indica Varieties Improved NPTs x Elite Indica Varieties Further Improvement in NPTs
IRRI: Planning Breeding Programs for Impact What crosses do you currently focus on in your institute?
IRRI: Planning Breeding Programs for Impact Types of crosses: Single crosses Backcrosses Three way/Top crosses Around 900 crosses yearly (wet & dry season) Cross designation (IR80369) F1 Nursery
IRRI: Planning Breeding Programs for Impact
F1
IRRI: Planning Breeding Programs for Impact Grow 2-6 rows (30 Plants) /cross Rouge off-types Identify accidental selfs Field Operations
IRRI: Planning Breeding Programs for Impact Protected: In general susceptible to diseases and insects Unprotected: In general resistant to diseases and insects First grow in blast nursery & Reject Susceptible populations Space planted 35 x 25 cm. Population size from 2,500- 5,000 individuals F2 Nursery
IRRI: Planning Breeding Programs for Impact Blast Nursery
IRRI: Planning Breeding Programs for Impact Blast Nursery
IRRI: Planning Breeding Programs for Impact Blast Nursery
IRRI: Planning Breeding Programs for Impact Protected F2
IRRI: Planning Breeding Programs for Impact Unprotected F2
IRRI: Planning Breeding Programs for Impact Inoculate with BB Race1/Race2 Rogue susceptible/undesirable plants Harvest desirable plants ( plants) Field Operations
IRRI: Planning Breeding Programs for Impact Four nurseries/year: January & July (Unprotected) May & November (Protected) Space planted 30 x 20 cm Every 20 th row is a check variety (4 Checks) 16,000 to 20,000 rows (30 plants) Pedigree nursery What system do you have in your institute?
IRRI: Planning Breeding Programs for Impact Pedigree Nursery in May
IRRI: Planning Breeding Programs for Impact Pedigree Nursery in May
IRRI: Planning Breeding Programs for Impact Pedigree Nursery in July
IRRI: Planning Breeding Programs for Impact Pedigree Nursery in July
IRRI: Planning Breeding Programs for Impact Screening requirements Memo
IRRI: Planning Breeding Programs for Impact Field Book
IRRI: Planning Breeding Programs for Impact F3 single rows F4 onwards 3/6 rows Selection between rows/within rows- at least 3 plants.
IRRI: Planning Breeding Programs for Impact Progeny Rows Composition of 2004 NOV PN GenCross typeNo. GenCross typeNo. F3I/I/I4 F5I/I5 IND/IND18 I/I/I2 IND/WH3 I/J/I18 JAP/BAS/ARO4 J/I6 JAP/I/I1 J/J3 Jap/Korean10 Micronutrient21 NPT/IND19 F6I/I17 NPT/JAP/IND3 NPT/I32 F4I/I11 NPT/NPT17 I/I/I1 J/I/I18 I/J31 Micronutrient40 NPT/BAS6 F7I/J2 NPT/I/I5 Micronutrient20 NPT/NPT14 NPT/NPT/I3
IRRI: Planning Breeding Programs for Impact CrossParentsStartEnd Sel. Gen. Type IR 79228IR F7I/J IR IR 79232IR F7I/J IR IR 79483IR F6I-NPT/NPT IR IR 79484IR F6I-NPT/NPT IR IR 79485IR F6I-NPT/NPT IR IR 79487IR F6I-NPT/NPT IR IR 79489IR F6I-NPT/NPT IR IR 79493IR F6NPT/NPT IR IR 79494IR F6NPT/NPT IR NOV PN
IRRI: Planning Breeding Programs for Impact OYT
IRRI: Planning Breeding Programs for Impact RYT
IRRI: Planning Breeding Programs for Impact Any questions or comments?