Dr H Schnerr Leatherhead Food International Ltd., Randalls Road, Leatherhead, Surrey, KT22 7RY  +44 (0)   Biosensor Technology An Innovative Option for the Food Sector

SPR Suppliers CompanyInstrumentWebsite Biacore Affinity SensorsIAsyshttp:// IBIS TechnologiesIBIShttp:// Texas InstrumentsSPREETAhttp:// Analytical µ-Systems BIOSUPLARhttp:// GWC technologiesSPRimagerhttp:// ReichertRefractometer- SR INIXhttp://

Objectives of the Experiment Concentration Analysis: How MUCH? Active Concentration Solution Equilibrium Inhibition Affinity Analysis: How STRONG? K D, K A Relative Ranking Yes/No Data Ligand Fishing Kinetic Rate Analysis: How FAST? k a, k d K D = k d /k a, K A = k a /k d

SPR technology Label-free Real-time Unique, high quality data on molecular interactions Simple assay design Robust and reproducible Walk-away automation Minimal sample preparation Rapid method

Binding Event Real-time Sensorgram Biomolecular Binding in Real Time

Principle of Detection  1   RU Time

Direct measurement Indirect measurement Inhibition in solution assay (ISA) Surface competition assay (SCA) Direct Binding Assay (DBA) Flexibility in Assay Design

Assays Relevant to Industry Food Quality Vitamins Biotin Folic Acid B 12 Pantophenic Acid (B 5 ) Authenticity Nonmilk Protein in Milk Powder GMO Testing Process-dependent Food Change Pasteurisation of Milk

Assays Relevant to Industry Food Safety Mycotoxins DON Aflatoxin Antibiotics, growth promoters and veterinary drug residues Streptomycin Chloramphenicol  -agonists Food Pathogens Salmonella E. coli O157:H7

Assays Relevant to Industry Food Related Studies Protein-Polysaccharide-Interactions  -casein and  -/  -carrageenan Binding Interaction Studies of Food Pathogens Salmonella and Collagen/Muscle Proteins E. coli O157:H7 and Collagen Interactions with membranes, receptors etc. Looking for Bioactive Appetite Suppressants

Assays Relevant to Industry Life Science/Drug Discovery Proteomics Ligand Fishing SPR/MS Antibody Characterisation Isotyping Affinity Kinethic Target ID Elucidate Disease Mechanisms Define Drugs or Diagnostic Markers

Sensor Development - Biotin/Folic Acid Pathogen Detection - Salmonella Allergen Testing Interaction studies - Polysaccharide - Protein Method Evaluation & Validation Routine Analysis - Qflex ® Kits for Vitamins LFI and Biosensors

SPR detects binding events as changes in mass at the chip surface Real-time kinetic measurements Label-free technology Qualitative rankings Flexibility in assay design Minimal sample preparation One platform for many different assays Summary

Biosensor Technology An Innovative Option for the Food Sector