Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering by Kenichi Nagase, Jun Kobayashi, and Teruo Okano Interface Volume 6(Suppl 3):S293-S309 June 6, 2009 ©2009 by The Royal Society

Schematic of surface modification methods using polymers: (a) EB irradiation, (b) ‘grafting to’ and (c) ‘grafting from’ approaches (MBAAm, N,N-methylenebisacrylamide) and (d) surface- initiated living radical polymerization (ATRP, atom transfer radical polym... Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

Concept of temperature-responsive chromatography: (a) hydration and dehydration of grafted polymer under an external temperature change ((i) hydrated, expanded PIPAAm and (ii) dehydrated, shrunken PIPAAm), (b) hydrophobic interaction with analyte and (c) ch... Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

Concept of temperature-responsive ion-exchange chromatography: (a) electrostatic interaction with analyte and (b) chromatograms ((i) 10°C and (ii) 50°C) of adenosine nucleotides separated on cationic copolymer-grafted silica beads. Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

Concept of temperature-triggered affinity control in the context of conformational change in PIPAAm molecules: control of (a) albumin adsorption/desorption and (b) asialotransferrin adsorption/desorption (Ricinus communis agglutinin). Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

First-generation temperature-responsive culture dish. Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

Concept of cell sheet engineering. Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

Harvested oral mucosal epithelial cell sheets. Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

Schematic of affinity control between integrin receptors and biomolecule-immobilized temperature-responsive culture dishes. Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society

Microfabrication process used to create thermoresponsive micropatterned surfaces. Kenichi Nagase et al. J. R. Soc. Interface 2009;6:S293-S309 ©2009 by The Royal Society