Pick up sample 1. 1.Add a tip 2. 2.Push to 1 st stop 3. 3.Lower tip into solution 4. 4.Release plunger Drop off sample 1. 1.Lower tip into tube 2. 2.Push.

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Presentation transcript:

Pick up sample 1. 1.Add a tip 2. 2.Push to 1 st stop 3. 3.Lower tip into solution 4. 4.Release plunger Drop off sample 1. 1.Lower tip into tube 2. 2.Push to 2 nd stop 3. 3.Raise tip out of tube 4. 4.Release plunger Add these to your tube: 42.5  l, 28.0  l, 37.5  l, 48.0  l and place in rack Put 30  l in wells 2-7 and place by microwave Assemble electrophoresis tray as shown

Sample Results

Pick up sample 1. 1.Get new tip 2. 2.Set to 30  l 3. 3.Push to 1 st stop 4. 4.Push tip to bottom 5. 5.Release plunger Drop off sample 1. 1.Lower tip into well 2. 2.Push plunger to 2 nd stop 3. 3.Raise tip out of well 4. 4.Release plunger 5. 5.Discard tip Load tray into chamber 1. 1.Wells to negative end 2. 2.Fill buffer above the gel 3. 3.Double stack

Hypercholesterolemia 1. 1.How do our cells use cholesterol? 2. 2.What is the problem with having too much LDL? 3. 3.What method(s) do we have for combatting a high LDL/HDL ratio? 4. 4.A mutated allele for a certain gene leads to FH. For what does the normal allele code?

Hypercholesterolemia 5. 5.What are the genotypes of Patients 1, 2, and 3? 6. 6.Explain how the following changes in procedure would affect results: a) a)Doubling the agar powder when making gels. b) b)Mixing up the connections to the power supply. c) c)Adding only half of the instructed sample into each well.