1 GENE TRANSFER AND GENETIC ENGINEERING CHAPTER 8.

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Presentation transcript:

1 GENE TRANSFER AND GENETIC ENGINEERING CHAPTER 8

2 Gene Transfer Vertical to offspring Lateral (horizontal) to others in same generation

3 Bacterial Gene Transfer and Recombinant DNA Technology Transformation –Griffith’s experiment

4 Transformation Mechanism Significance Recombination?

5 Transduction Mechanism Significance Transducing phages: p1, p22 Can be used in genetic mapping

6 Conjugation Mechanism Significance Also used for mapping Conjugation pilus

7 Plasmids F plasmid (conjugation) Resistance plasmids Virulence plasmids

8 Plasmids (cont.) Tumor-inducing plasmids Agrobacterium tumefaciens and plants Plasmids conferring novel catabolism Degradation pathways on a plasmid

9 Genetic Engineering Genetic fusions reporter fusions promoter fusions

10 Genetic Engineering Protoplast fusion electroporation

11 Genetic Engineering (cont.) Gene Amplification Recombinant DNA cloning

12 Applications Medical Industrial Agricultural Hybridomas

13 Risks and Benefits Frankenstein? Frankenfoods?

14 Recombinant DNA technology Plasmids - autonomous replicating extrachromasomal DNA restriction enzymes

15 Viral technology- phage cloning

16 Homologous recombination Used to make gene knockouts in bacteria and yeast

17 Transposons - jumping genes

18 Evidence for extensive transfer of antibiotic resistance genes in Bacteroides sp.