AGENDA Who Done It Workshop Continental Breakfast Welcome and Introductions Who Done It Lab Overview of SCBC Resources on MATSC Reserving Your Kit on LARS.

Slides:

Advertisements

Similar presentations

Salk Institute Mobile Lab Gel Electrophoresis Teacher Instructions Edvotek Set Up 12 groups.

Advertisements

TOOLS AND TECHNIQUES DNA Fingerprinting. Intoducing the microLiter! A TINY amount…a millionth of a Liter Very difficult to measure because it is SOOO.

Salk Institute Mobile Lab Gel Electrophoresis Student Instructions VWR Set up.

Stage 1: Prepare your plasmids to be cut by restriction enzymes Obtain the plasmids (pKAN and pAMP) P stands for plasmid pKAN = plasmid with antibiotic.

Loading Gels. The Gel Box Electrode Lid Tray to hold gel Connect to power supply.

Using a Micropipette Huntington Gardens July 2013

An Introduction to Microvolumetrics and Pipetting

Salk Institute Mobile Lab Gel Electrophoresis Teacher Instructions VWR Set Up 12 groups Mira Costa kit.

Lab 9. Human Mitochondrial Analysis using PCR and Electrophoresis Major Goals of this Experiment Isolate mitochondrial DNA (mtDNA) from cheek cells and.

Casting an Agarose Gel Tricks of the Trade. Setting up the Casting Tray  Put the casting tray on level, non- movable surface in a place where it won’t.

CHAPTER 1: Some Tools of the Trade Lab

DNA-Fingerprint1 Procedure of DNA-Fingerprints. DNA-Fingerprint2 Tubes for each workgroup.

DNA Structure and Analysis Activity 4.2: Casting Agarose Gels.

BRIDGES 2014 Agarose Gel Visualization of Restriction Enzyme Digest.

Outbreak Lab: In this lab, biotech procedures will be used to see if a sample of viral DNA is the deadly Alabama virus. The specific technique that you.

Separation of Proteins by Gel Electrophoresis Carolina Biological Kit #

Prepered by:- Rana Al-Turki

Salk Institute Mobile Lab Gel Electrophoresis Teacher Instructions BioRad Set Up 8 groups Grossmont Kit.

DNA-Fingerprint1 Detection of PCR Products by Agarose Gel Electrophoresis.

BIO-RAD Forensic DNA Fingerprinting Kit

Agarose Gel Electrophoresis

DNA Analysis Using Agarose Gel Electrophoresis Day 1

Collect Buccal Cells. PCR Polymerase Chain Reaction DNA/gene amplification.

DNA Structure and Analysis Activity 4.3: Dye Electrophoresis.

Electrophoresis Electrophoresis is the movement of molecules by an electric current .This is practically done in a matrix to limit migration and contain.

LABORATORY 1: TOOLS OF THE TRADE LSSI Alum, 2009 Jennifer Muñoz, Del Mar Union School District.

Introduction to Gel Electrophoresis. Outline How to prepare a gel How to micropipet Practice setting up electrophoresis Discussion viewing of gel –In.

GEL ELECTROPHORESIS. FIRST, WHAT……  Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according.

Wildlife Forensics Environmental Study Center. Chinchilla.

Gel Electrophoresis. Definition – COPY ME! Separation of DNA fragments according to size and charge Based on movement through a gel medium when an.

BIO-RAD Lambda DNA Kit, and BIO-RAD Forensic DNA Fingerprinting Kit Section Concepts: DNA Scissors Activity, restriction enzymes, gel electrophoresis,

Agarose gel electrophoresis. Agarose gel electrophoresis is an easy way to separate DNA fragments by their sizes and visualize them. It is a common diagnostic.

Gel Electrophoresis.  This workforce solution was funded by a grant awarded under the President’s High Growth Job Training Initiative as implemented.

Gel Electrophoresis of DNA. DNA as Forensic Evidence Individual evidence – identify a single person Trace evidence – small amount left at crime scene.

Program Introduction Chapter 1 – Some Tools of the Trade.

CHAPTER 1: Some Tools of the Trade Lab Purpose of Lab 1.1 Become familiar with the small volumes of solutions used in molecular biology Introduce.

How to use a micropipette. When is a micropipette needed? Micropipettes are precision instruments designed to measure and transfer small volumes of liquids.

1. Which tool would you use for picking up 15 microliter? Which tool for 25 ml? Why? 2. Why do the little test tubes look this way? 3. Answer “Micropipette.

Gel Electrophoresis.

DNA Fingerprinting Agarose Gel Electrophoresis Student Instructions

Gel Electrophoresis What is Gel Electrophoresis? Gel electrophoresis separates molecules on the basis of their charge and size. The charged macromolecules.

Lab.3 Gel electrophoresis

Proteomics Day 3 Tech Talk. Activities 1.Prepare IPG strip for second gel electrophoresis 2.Run IPG strip in second dimension SDS- PAGE electrophoresis.

One test used in forensic labs is DNA fingerprint. It is also called a DNA profile. Analysts use the DNA profile from potential suspects and compare it.

DO NOW 1. Get out your Embryos-R-Us HW 2. Detail the decision you made. Why did you make that decision?

Proper Micropipette Technique and Slide Preparation

Part Power DNA How fast will the DNA migrate? strength of the electrical field, buffer, density of agarose gel… Size of the DNA! *Small DNA move.

Gel Electrophoresis. What is Gel Electrophoresis? Electrophoresis: Movement of charged particles through a solution (gel) under the influence of an electric.

Bellwork 1. What do you think the word gel electrophoresis means? 2. Who can move most quickly through a thick, dense forest: a human, a dog, or a rabbit?

ABE Labs 1.1 and 1.2 Tools of the Trade. CHAPTER 1: Some Tools of the Trade Lab

Build-a-Gene X Edit. Two projects Traditional method – Using site-directed mutagenesis to edit a gene – Make red fluorescent protein brighter – Weeks.

LABORATORY 1: TOOLS OF THE TRADE

An Introduction to Microvolumetrics and Pipetting

DNA Fingerprinting One test used in forensic labs is DNA fingerprint. It is also called a DNA profile. Analysts use the DNA profile from potential suspects.

Outbreak Lab: In this lab, biotech procedures will be used to see if a sample of viral DNA is the deadly Alabama virus. The specific technique that you.

Gel Electrophoresis of DNA

Gel Electrophoresis By: Sariah Arnold.

Gel Electrophoresis Teacher Instructions BioRad Set Up 12 groups

Inquiry into gel electrophoresis

Gel Electrophoresis Method of separating molecules within an electric field based on the size and charge of DNA fragments.

Introduction to Gel Electrophoresis

Introduction to Gel Electrophoresis

An Introduction to Microvolumetrics and Pipetting

Agarose gel Electrophoresis

Crime Scene DNA Profiling Lab

Introduction to Gel Electrophoresis

Agarose Gel Electrophoresis

Opening Activity: March 20, 2017

Forensic DNA Fingerprinting:

Presentation transcript:

AGENDA Who Done It Workshop Continental Breakfast Welcome and Introductions Who Done It Lab Overview of SCBC Resources on MATSC Reserving Your Kit on LARS Feedback

Agenda Forensics Overview Pipetting: video, steps in procedure, practice, and assessment Gel Electrophoresis Part 1: video, card sort, and prepare agarose gels (follow the SOP) Practice pipetting and loading gel wells Prepare your Samples Gel Electrophoresis Part 2: load samples and run gels Analyze results and determine identity of criminal Performance Assessment: Creating a Standard Curve to identify an unknown

DNA Day April 14 th 2009 DNA Day

National Human Genome Research Institute: DNA Day April 14 th 2009 DNA Day

Who Done It? Before you can put your Crime Scene Investigator hat and determine WHO committed the crime, you must first lean need to how to pipette and load dyes into a gel.

The Basic Laboratory Tool: Micropipette

Part I - An Introduction to Pipetting gen.usingmicro/

How do you use a micropipette? (Prepare)

When using a Pipette raise the tube to eye level and remember: Number in red represents the decimal place for the P-20 Use the knob on the handle to change the volume Always use the pipette with a tip Feel the pressure difference between first stop and second stop First stop aspirates (draws up the solution) Second stop dispenses (pushes out) the solution

Learning a New Skill: Micropipetting (Practice)

Setting the Volume on a Micropipette: Think (Assessment) 1)The smallest amount of volume that I can measure with a P-20 pipette is… 2) When I use a P-20 pipette the maximum volume I can measure is…

Thoughts?

Gel Electrophoresis

Making an Agarose Gel agarose-gel//

Electrophoresis and Gel Analysis analysis/

Follow the SOP to Melt and Pour the Agarose Gel

Place in the microwave

It is ready to pour when it is cool to the touch… The solution should become clear…

Use the 6 well side of the Comb

Pour the agarose and let it solidify Remove the comb after the gel has solidified

Gel Electrophoresis Card Sort

Part II: Preparing the Samples

Practice Loading Dye into a Gel: Fill with 1X SB Buffer

Place the gel tray in the chamber with the wells closest to the negative end Pour buffer into the chamber so that it covers the gel completely

Load the wells, be careful not to poke a hole…

Place the hood on top of the chamber and press run button on the carriage Let it run for minutes

Part III: Analyze the Results and Determine who Committed the Crime

CSS2VS1 Unknown

Part IV: Use mathematics to Create a Standard Curve and Identify an Unknown (Performance Assessment)