Presentation of AdvanCE FS96 for High Throughput DNA Fragment Analysis 1.

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Presentation transcript:

Presentation of AdvanCE FS96 for High Throughput DNA Fragment Analysis 1

Our history Twelve year old privately-held instrumentation company 35 employees Over 120 instrument in operation worldwide Our business commitment: Introduce innovative technologies Build strong customer relationships Provide excellent technical support and services Our instrument solutions Rapid microbial detection and enumeration Capillary electrophoresis instruments designed for specific applications Advanced Analytical 2

Current instruments Micro PRO™ - Flow cytometry based system designed for rapid microbial detection and enumeration. pKa PRO™ - Multi-channel parallel CE for rapid measurement of acid dissociation constants (pKa values) of water soluble and insoluble drug compounds. Oligo PRO™ - Multi-channel parallel CE for size-based purity analysis of single stranded DNA and RNA oligonucleotides, and double stranded RNA interference (RNAi) products. Protein PRO™ - Medium throughput parallel CE protein analysis system, capable of both size (CGE) and charge (CZE) separation. AdvanCE FS™ - Multi-channel capillary electrophoresis fluorescence detection for DNA, carbohydrates and protein analysis. Full line of consumable products for each instrument 3

Instrument placements Micro PRO US Army Pfizer Wyeth Amgen Medimmune Intervet (UK) Boehringer Ingelheim Pfizer Animal Health Alberto Culver Procter & Gamble Vistakon (J&J) pKa PRO & Oligo PRO Pfizer Merck Roche Sanofi-Aventis BASF EGEA Biosciences (J&J) Invitrogen Integrated DNA Technologies Illumina 4

Patents Integrated Multiplexed Capillary Electrophoresis System Using Absorption Detection. – US Patent No. 6,387,234. Issued May 14, 2002 RBD Sample Delivery Methods, Key to Low-level Detection. – US Patent No. 6,473,171. Issued October 29, 2002 Method of Analyzing Multiple Samples Simultaneously by Detecting Absorption. – US Patent No. 6,788,414. Issued September 7, 2004 Multiplexed, Absorbance-Based Capillary Electrophoresis System and Method. – US Patent No. 6,833,062. Issued December 21, 2004 Multiplexed, Absorbance-Based Capillary Electrophoresis System and Method. – US Patent No. 6,833,919. Issued December 21, 2004 Two-Dimensional Protein Separations Using Chromatofocusing and Multiplexed Capillary Gel Electrophoresis. – US Patent No. 6,969,452. Issued November 29, 2005 Capillary Electrophoresis Gel Especially for Separation Made for Single Stranded Nucleic Acid Separations. – US Patent No. 7,083,711. Issued August 1, 2006 Robotic Friendly External Loading System for Electrophoresis Instrument and Method. – US Patent No. 7,118,659. Issued October 10, 2006 Method for Reducing Background Fluorescence. – US Patent No. 7,205,100. Issued April 17,

A DVAN CE FS96 FLUORESCENCE SYSTEM Advanced Analytical 6

Capillary Gel Electrophoresis (CGE) CGE provides size-based resolution separations of DNA fragments Resolution is dependent on gel and DNA fragment size. Size differences (5bp) of smaller fragment (<500bp) are more easily resolved. Fragments larger than 1000bp will have less separation therefore less resolution is achieved, mainly because large fragments move morer than small fragments. Gel matrices can be designed to resolve small difference in the fragment size but range becomes limited. AATI gels for DNA fragment analysis contains a highly sensitive fluorescent dye that intercalates dsDNA. The LED emits at 470nm; detection is at +500nm CGE also provides low sample consumption and automated operation -+ Fluorescence

Permanent vs. Dynamic Capillary Wall Coating The capillary wall contains charged silanol groups pH > 4, creating an ionic double layer that generates bulk fluid flow (electro osmotic flow or EOF) from the anode (+) to the cathode (-). The EOF is opposite to the migration of DNA, and can cause a loss of separation efficiency and increase in migration times How to Eliminate EOF? Permanent coatings involve chemical bonding of molecules to the capillary wall. They are non-replaceable and tend to have a limited lifetime. Dynamic coatings physically bond to the capillary wall by hydrophobic or charge forces. They are replaceable and have a long lifetime when periodically re-conditioning the capillary walls. Dynamic coatings are preferred for their lifetime and ease of use

AdvanCE FS96 System A dedicated 96-channel CGE system optimized for high throughput DNA fragment analysis Rapid separation of DNA fragments and plasmid DNA Simplified user interface with predefined methods for ease-of-use and streamlined operation Enhanced software features, data analysis and report generation capabilities LED based fluorescence 9

Principles of Parallel CE – LED Fluorescence Operation 96 capillaries are arranged in a linear array at detection window Fluorescent light excites the intercalated dye; emisson is measured by a CCD detector Capillary inlets are arranged 8 x 12 for direct sample injection from 96-well micro plates Capillary outlets are bundled and connected to a high pressure pump for gel matrix filling Samples are simultaneously injected by voltage 96 individual CGE separations are performed in parallel CCD detector LED fluorescence 10

High Pressure Pumping System Separation Gel Matrix A/B Switching Valve Up to 400 psi can be applied for flushing the capillary array CE grade Water 11

LED Fluorescence vs Laser Induced Fluorescence LED fluorescence Long life – hours Low maintenance Low replacement cost Laser induced fluorescence Short life span (2,000 hours) High replacement cost – – € Requires regular maintenance of gas and alignment

AdvanCE™ FS96 Operational Flow Chart Step 1. Flush Capillaries with Gel 10 minutes at 300 psi Step 3. Injection and Separation for 96 Samples 30 – 70 minutes Step 2. Pre-run to stabilize system 1 minute at separation voltage Step 4. Flush capillaries 5 minutes at 300 psi Repeat Steps 2 through 4 a total of 10 times then flush and replace reservoir with fresh gel and re-condition capillaries

Sample Throughput:96 samples – 2 plates can be run unattended Detection:Online, LED based fluorescence, 700 mW, 470 nm excitation, collection above 500 nm with CCD camera Sample Injection:Simultaneous electrokinetic injection from a 96-well microplate Power supply:20 kV negative polarity power supply Cooling:Peltier cooler Sensitivity:5 pg/µl without the need to desalt Sample Format:DNA fragments in buffer or water. Sample Volume Required:Minimum volume 20  l/well Software:Proprietary AdvanCE software for system control/data analysis Data Export Format:Microsoft ® Word or PDF reports for individual samples or entire sample set Environmental Conditions:Indoor use, normal laboratory environment; lab temperature 15–25º C Relative Humidity Range:< 80% (non-condensing) Electrical:100–200 VAC; Hz (200–230 VAC; 50–60 Hz available); 15 A Instrument Dimensions:Fully configured requires 96” W x 30” D x 39” H Instrument Weight:195 lbs. (88.6 kg) AdvanCE™ FS96 Specifications

Direct parallel injection and separation of an entire plate at once Fast run times to increase sample throughput Easily separates all fragments over important DNA range ( bp, bp, bp) No need to desalt sample prior to injection, detect low quantity fragments Low per sample cost Flexibility, flexibility, flexibility – variable capillary dimensions and lengths, transfer methods directly from single cap system Three gel matrices – highly accurate gels to resolve fragments from 10-12,000bp and plasmids Multiple ways to view fragments – speeds analysis and report generation Key Benefits of the AdvanCE™ FS96

Key Features of the AdvanCE™ FS96 96 capillary array – new design Short run times – separate <1000bp fragments in 30 minutes 5bp resolution 500 – 1,000 bp 5 pg/  l sensitivity Low cost/sample Variable capillary dimensions Variable gels User friendly software

DNF – Best for small fragment analysis, 10 – 300 bp. Gel resolution of 3-5 bp DNF – Broad range PCR fragment gel; analyze fragments 50 – 2,000 bp. Gel resolution varies from 5bp 500bp,+100bp >1000 DNF – Large and medium size fragment analysis, 1,000 – 12,000 bp. Gel is also capable of separating major plasmid DNA species, supercoiled, relaxed and linear species. Gel types available for FS system

DNF – dsDNA inlet buffer – 1 L DNF – Capillary conditioning solution – 1 L A P – Short CAC box for DNF and DNF A P – Long CAC box for DNF and DNF Other components for FS system

Sample: 100 bp ladder Method: Injection 5kV for 5 seconds, voltage 8kV, capillary 75  m x 33cm/55cm DNA fragment separation

Sample: PCR product diluted with water 5 times Method: Injection 5kV for 5 seconds, voltage 8kV, capillary 50  m x 33cm/55cm PCR fragment separation

96-Capillary Separation 96 different samples analyzed simultaneously

55cm/80x50  m, 5kv for 10s 7kV injection Large dsDNA fragments analysis

Quantify and size fragments simultaneously

Sample: pBR322 plasmid DNA (2  g/ml in buffer), supercoiled, digested and nicked Method: Injection 2kV for 5 seconds, voltage 7kV, capillary 75  m x 33cm/50cm Plasmid separation 24

5 pg/  L 10 pg/  L 20 pg/  L 40 pg/  L 80 pg/  L 160 pg/  L 320 pg/  L Close up 5pg/  l S:N >10:1 Which level of sensitivity would you choose?

Which resolution would you choose? Qiaxcel

Which resolution would you choose? Qiaxcel AdvanCE FS96

The analytical software is an integral part of the system and is designed to quickly analyze the samples. A results in a data file can be viewed multiple ways including a digital image that looks like a traditional agarose gel, by flagging, individually or in groups as selected by user. The report generation screen allow for multiple formats Examples screen shots below. PRO-Size Analytical Software

Summary Most flexible multi channel fluorescent instrument on the market. Vary capillary dimensions and length No sample preparation (desalting step) is required for analysis times more sensitive than other systems Gels have high separation resolution over a wide DNA range Three separate gels capable of resolving fragments from 10-12,000bp, including a gel for plasmid DNA Transfer methods directly from single capillary system Easy to use software, produces digital images and predicts both size and relative quantity of fragments

Thank you Contact : William Amoyal Disruptive Technologies (distributor France, Belgium, Spain) 3 allée des Camélias Villecresnes Tél Web 38