Purdue University Ann Rundell, Assistant Professor Workshop on BME Teaching of Innovation, Design & Entrepreneurship

Program Objectives New Undergraduate Program, Fall 2004 Teach engineering science, analysis, and design in the context of biology and biomedical problems Immerse students in key life science principles early Integrate biology and engineering within courses Emphasize problem-solving, design, and teamwork Allow for senior-level specializations Incorporate ABET considerations throughout Purdue University

Become familiar with the classes of molecules that form the cellular components of living organisms (biomolecules) –sugars, lipids, proteins, and nucleic acids Relate structure and function of these important classes of molecules Hydrogen-bonding, hydrophobic forces, electrostatic interactions along with other weak interactions Understand how these concepts are integral to engineering, controlling and manipulating biomedical platforms Purdue University Biomolecules: Structure Function and Engineering Applications

Laboratory will teach and introduce key skills, techniques, and analysis tools to support the cells and biomolecules courses Purdue University Biomedical Engineering Laboratory I: Exploring Biomolecules and Cells Module 1: Fundamental Quantitative Techniques Module 2: From Biomolecules to Cells: Isolation, Identification and Quantification Module 3: Analysis of Integrated Biosystems Module 4: Application and Design of Biomedical Platforms Engineering Fundamentals Biomolecules Concepts Cell Basics BME Lab I

Describe particularly novel and/or effective methods you use to teach innovation & entrepreneurship Implement series of mini-design projects throughout curriculum with capstone design experience senior year Sophomore Mini-Design Project –DNA and Protein Chips –Students demonstrate fundamental principles of biosensor design –Design to be tested via simulation prior to fabrication –Build DNA or Protein Chip in laboratory and test Purdue University

How do you measure outcomes? Design: –Formal design reviews with invited industrial representatives –Student demonstrations of working aspects of design projects Concepts: –Virtual Laboratory on Web A virtual laboratory bench with all of the equipment available in the lab Students will be presented with a task and must identify equipment used for task at hand, provide a reason for its inclusion, sequence the steps to perform the task Provides immediate feedback with ability to iterate until the student successfully completes the task Provide feedback to instructor on how long it took the students to complete the assignment, how many iterations it took, and compile a list of common problems –Written assignments: Homework, quizzes and exams Purdue University

Describe a course project or exercise that exemplifies “best practices” Sophomore Lab on Polymerase chain reaction (PCR) Introduces primer design, PCR amplification, and quantitative measurements of nucleic acid material –Prelab: Rank selected oligonucleotide primers by their optimal annealing temperatures using computer programs. Predict the quantity of nucleic acid material present after different numbers of cycles. –Lab: Determine the composition of unknown samples of nucleic acid material by applying their knowledge of primer design, benchtop PCR thermocyclers, agarose gel electrophoresis, and UV spectroscopy. Primer design, PCR, and quantitative analysis of genetic material will be essential skills for future biomedical engineers for the manipulation and analysis of genetic material Purdue University