(RFLP Electrophoresis)

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Presentation transcript:

(RFLP Electrophoresis) DNA Fingerprinting (RFLP Electrophoresis)

Four nucleotide bases make up the chains of the double helix DNA Double Helix Four nucleotide bases make up the chains of the double helix

RFLP (Restriction Fragment Length Polymorphism) DNA Fingerprinting RFLP (Restriction Fragment Length Polymorphism)

1 microliter (ul) = 10-6 liters = 0.000001 liters 1 millionth of a liter

Restriction Enzymes cut DNA at specific recognition sequences – typically palindromes. Restriction enzymes are also called restriction endonucleases and the process of cutting the DNA is called a restriction digest .

DNA samples with the EcoR1 and Pst1 recognition sequences at different places will be cut into fragments of different lengths. Fragments can then be separated by size to create an RFLP banding pattern using Gel Electrophoresis.

DNA is negatively charged so the fragments are drawn through the agarose gel towards the positive electrode. Large fragments move more slowly, smaller fragments move more quickly. The pool of DNA fragments separates into discrete bands creating an RFLP DNA Fingerprint.

Gel Electrophoresis

Negative (-) Electrode Positive (+) Electrode CS S1 S2 S3 S4 S5 WELLS (lanes) Positive (+) Electrode