M201 Microplate Reader This power point file is prepared mainly for the engineers of our distributors.

Advice for this course You needn’t make a detailed note, especially with the diagrams. You can copy and keep this file, then review it anytime. Pay more attention to my explain can help you understand it clearly. Some diagrams are truly hard to draw by hand ^_^. There will be a short pause after each section. You can ask some questions about that section then, I would do my best to answer you. Please remember the email of the product specialist: ivdservice@china-emperor.com, if you have some further questions, suggestions, please send an email.

Basic principle Antigen will combine only with the specific antibody

ELISA test Antibody Plate Well Antigen (from sample) Enzymes Enzymes Substrate Colored product catalyzing

Steps combine catalyzing combine wash wash Stop buffer wash Read Antibody wash Sample wash catalyzing Stop buffer Substrate wash Antibody-Enzyms combine Read

Advantages Mono-LED light source Based on Windows CE.net 7” TFT colorful LCD screen. Optional touch screen supported. Membrane keyboard with numeric keys and short-cut function keys. Multi-test (different tests on one plate) Test, calibrate, QC on one board Fast reading (less than 5s for whole plate) Large memory:1200 plates (96 wells).

(bottom side) LED board Inner view Keyboard 7” LCD display Printer board Main fan Core board Back end board Power supply 仪器的构成 上盖上面的部件主要为显示器，键盘和打印机，都是常见的输入输出设备，在此不做介绍 拆掉上盖后就能看到这张图片所示的内部结构： 主要有 电源盒，主风扇，前端板，后端板，核心板，转接板，泵，光源模块，比色模块，滤光模块等 Transfer board Plate tray (bottom side) LED board motor & detectors Front end board

Functional Block Diagram 1 LED Array 8x4 (+2) Keyboard Light sensor x8 LED motor Plate motor Front-end Union LED steps detector Plate steps detector LED origin detector Plate origin detector Control signals from back end

Functional Block Diagram 2 Data from front end light intensity and detector signal, etc. Touch screen PC External Keyboard LCD and buzzer Back-end union Mouse Thermal printer Barcode reader Standard PCL printer USB disk SD card

LED Array 405 450 492 630 Optional x2 8 Channels Patent: mono-LED illumination. More than 100,000 hours long life. Needn’t replace lamp any more. No stabilizing time. Device can be fast turned on. Twinkling reading. Low power and low heat.

Wavelength control Front-end board LED Array Opto-coupler 珀尔贴元件示意图 我们可以通过切换电压方向来让升温或降温。 Opto-coupler Origin detector Opto-coupler Step detector

Microplate EMP-168兼容比色池和比色杯两种液体模式 比色池模式有以下优势 操作简单，操作时不需要频繁更换； 寿命长，比色池和杯座都能使用较长时间 经济，使用少量的试剂和样本就能进行测量

Plate position control Opto-coupler Step detector Front-end board Hall element Origin detector

Breakdown Description Troubleshooting 1 Breakdown Description Solutions Device can not start check the power plug check the fuse check the power supply Cannot pass Self-check make sure the plate carrier is empty restart the machine check the optical path Results abnormal 1. check if the plate lay correctly . check the test parameters detect the standard material Plate cannot move check if the plate is in position check if there is something on the block check if the plate carrier can move manually The results of individual channel abnormal check weather the result is normal when not put micro perforated panel check if there are some lens loose check if any LED damaged Mouse cannot move reconnect the mouse test the mouse on PC 常见故障解决办法，这里的内容在我们的服务手册上可以找到。 部分故障的具体解决办法，参考我们编制的指导文件。

Breakdown Description Troubleshooting 2 Breakdown Description Solutions Thermal printer cannot work Make sure the internal printer be selected in system setting Check if the printer paper is installed External printer cannot work make sure the internal printer be selected in system setting check if the USB cable connected well check if the printer type can be supported by machine Screen is blank with back light on check if the power equipment started property check the power supply check the FFC cable Keyboard cannot work check it he keyboard cable connection check if the voltage in frond-end normal Motor make noise check the plate carrier check weather the detection opocouple board loose check if there is some foreign matter

Please read user’s manual and service manual for more information. Thank you! Copyright © Shenzhen Emperor Electronic Technology Co., Ltd. 1st edition, June, 2012