Clindamycin induction test in treating patients infected with methicilin resistant Staphylococcus aureus Presented by Iyad Kaddora

Staphylococcus aureus Belongs to Micrococcaceae family. Belongs to Micrococcaceae family. Gram positive cocci. Gram positive cocci. Clusters resembling grape. Clusters resembling grape. Part of the humans normal flora. Part of the humans normal flora. Exist in air and water. Exist in air and water. Number one cause of nosocomial infections. Number one cause of nosocomial infections. Approximately 25% to 30% of the population is colonized. Approximately 25% to 30% of the population is colonized.

Staphylococcus aureus images Health info. 2005

S. aureus Virulence Factors Surface proteins Surface proteins Invasions Invasions Surface factors Surface factors Biochemical properties Biochemical properties Exotoxins Exotoxins Inherent and acquired resistance to antimicrobial agents Inherent and acquired resistance to antimicrobial agents molbio.princeton. university

40% of nosocomial S. aureus infections are methicillin resistant First reported in 1960s First reported in 1960s Causes severe morbidity and mortality worldwide Causes severe morbidity and mortality worldwide Endemic in many European and American hospitals Endemic in many European and American hospitals Many in-patients are colonized or infected Many in-patients are colonized or infected 25% hospital personnel may be carriers 25% hospital personnel may be carriers Spread by hand, usually of health Spread by hand, usually of health care workers

Risk factors for methicilin resistant Staphylococcus aureus colonization Advanced age Advanced age Male gender Male gender Previous hospitalization Previous hospitalization Length of hospitalization Length of hospitalization Chronic medical illness Chronic medical illness Prior and prolonged antibiotic therapy Prior and prolonged antibiotic therapy Presence and size of a wound Presence and size of a wound Exposure to colonized or infected patient Exposure to colonized or infected patient

Proportion of nosocomial MRSA among the intensive care unit patients NNIS System.

Rates of hospital MRSA isolates from 324 geographically distributed US health care institutions in year 2003 Pfizer. © 2005 Pfizer Inc.

Antibiotics Resistance Antibiotics are used to treat bacterial infections, but recently they start becoming less effective. Antibiotics are used to treat bacterial infections, but recently they start becoming less effective. In the past few years S. aureus has began to show more resistant to commonly used antibiotics. In the past few years S. aureus has began to show more resistant to commonly used antibiotics. S. aureus has developed new strains called methicilin resistant Staphylococcucs aureus (MRSA). S. aureus has developed new strains called methicilin resistant Staphylococcucs aureus (MRSA). Methcilin resistance now refers to multi- antibiotic resistant group.

Clindamycin antibiotic is drug of choice for treating patients infected with MRSA Clindamycin β-lactam antibiotic. β-lactam antibiotic. Inhibits the synthesis of protein in bacterial organism. Inhibits the synthesis of protein in bacterial organism. Prevents the bacteria from replicating. Prevents the bacteria from replicating. The failure of clindamycin treatment to MRSA infected patients, raises a big concern to health care professional and attending physicians. The failure of clindamycin treatment to MRSA infected patients, raises a big concern to health care professional and attending physicians.

Antibiotics resistance mechanisms The resistance to erythromycin and clindamycin occurs by one of two mechanisms. The resistance to erythromycin and clindamycin occurs by one of two mechanisms. Efflux: typically mediated by msrA gene. Efflux: typically mediated by msrA gene. Ribosome alteration: occur through methylation of the ribosomal target site, and this resistance is mediated by erm gene. Ribosome alteration: occur through methylation of the ribosomal target site, and this resistance is mediated by erm gene.

Objective: Many studies have been performed regarding MRSA. Many studies have been performed regarding MRSA. New test has been developed to test the future resistance of MRSA strains to clindamycin. New test has been developed to test the future resistance of MRSA strains to clindamycin. The hypotheses: MRSA strains are becoming more resistant to clindamycin during 2004/2005 year compared to 2000/2001. The hypotheses: MRSA strains are becoming more resistant to clindamycin during 2004/2005 year compared to 2000/2001.

Materials and methodology The testing specimens were collected in year 2000/2001, and year 2004/2005. The testing specimens were collected in year 2000/2001, and year 2004/2005. The specimens were saved in order to be evaluated for clindamycin resistance. The specimens were saved in order to be evaluated for clindamycin resistance. D test is performed on MRSA strains from sources other than urine. D test is performed on MRSA strains from sources other than urine. MRSA has to be erythromycin resistant and clindamycin sensitive. MRSA has to be erythromycin resistant and clindamycin sensitive.

The D Test D test is way to detect inducible clindamycin resistance. D test is way to detect inducible clindamycin resistance. In order for the resistance to show up inducing agent is required. In order for the resistance to show up inducing agent is required. D test output: D test output: 1. Positive D test. 1. Positive D test. 2. Negative D test. 2. Negative D test.

D-test principle D test is done to allow erythromycin induce production of methylase. D test is done to allow erythromycin induce production of methylase. Erythromycin ribosome methylase is a gene that encodes enzymes which confer inducible or constitutive resistance to clindamycin. Erythromycin ribosome methylase is a gene that encodes enzymes which confer inducible or constitutive resistance to clindamycin. Induced clindamycin resistance is detected by forming a D shape letter on the agar plate. Induced clindamycin resistance is detected by forming a D shape letter on the agar plate.

D-test positivist on the original wound culture isolate from the case patient. E, erythromycin; CL, clindamycin. Clindamycin disk Erythromycin disk A flattening of the zone in the area between the two disks (letter D shape) will indicate the organism’s ability to induce clindamycin resistance in the future. “D” Clinical Infectious Diseases 2003;37:

D test procedure: Make 0.5 McFarland suspensions (turbidity measurement) in3 ml sterile inoculum’s water. Make 0.5 McFarland suspensions (turbidity measurement) in3 ml sterile inoculum’s water. Sterile swab is used to inoculate Sterile swab is used to inoculate the organism to blood agar plate. the organism to blood agar plate. Place an erythromycin disk 15 to Place an erythromycin disk 15 to 26 mm away from the clindamycin disk, in the blood agar plate. Incubate hours at 35°C. Incubate hours at 35°C.

Disk induction testing. (A)Constitutive clindamycin resistance. (B) Negative disk induction test indicating the absence of inducible clindamycin resistance. (C) Positive disk induction test indicating inducible clindamycin resistance. Journal of Clinical Microbiology, April 2005, p , Vol. 43, No. 4

Results of the D test Clinical Infectious Diseases 2003;37: All clinical S. aureus Isolates at the Johns Hopkins Hospital - Nov 1, 2002 through Dec 17, Total S. aureus Isolates

Clindamycin Susceptibility Testing for Adult & Pediatric S. aureus Isolates Discordant Susceptibility Adult: 36% 17% iMLS Pediatric: 32% 20% iMLS Clinical Infectious Diseases 2003;37:

Clindamycin Susceptibility Testing for MRSA Clinical Infectious Diseases 2003;37:

ReportLocation (Pediatric vs. Adult) Subject for D-test PositiveD-test Como-Sabetti IDSA 2002 #92 Minnesota (Not specified) 64% of all MRSA84% Frank PIDJ2002;21:530 Chicago (Pediatric) 38% of all MRSA94% SattlerPIDJ2002;21:910 Houston (Pediatric) 90% of all MRSA8% Current Study Baltimore(combined)Ped:25% Ad: 44% 43%51% Reported Frequency of In Vitro Inducible Resistance to Clindamycin in MRSA Clinical Infectious Diseases 2003;37:

Clindamycin treatment of MRSA infections   31/33 were D test positive   9/31 treated with clindamycin 1 could not be evaluated 3 received multiple antibiotics 5 treated with clindamycin only 3/5: minor skin infections resolved on clindamycin 2/5: clinical failures on clindamycin Frank PIDJ 2002;21:530.

Lab Protocol for S. aureus S. aureus cultured MRSA Clinda R or Eryth S/Clinda S MSSA Clinda S and Erythro R Routine D-testD-test at request of Physician Report Clinda MICReport Clinda=R Clinda S and Erythro R Clinda R or ErythS /Clinda S Report Clinda MIC ++-- Report Clinda=RReport Clinda MICReport Clinda=RReport Clinda MICReport Clinda=RReport Clinda MIC

Clindamycin Treatment of S. aureus Infections MRSA MRSA Important therapeutic option if Important therapeutic option if Erythromycin and clindamycin sensitive Erythromycin and clindamycin sensitive Erythromycin-resistant, clindamycin sensitive IF D-test negative Erythromycin-resistant, clindamycin sensitive IF D-test negative MSSA MSSA Beta lactam more widely used Beta lactam more widely used Concern for treatment failure same as for MRSA, if clindamycin S, erythromycin R, D test positive Concern for treatment failure same as for MRSA, if clindamycin S, erythromycin R, D test positive

Clinical Significance of In Vitro Inducible Resistance to Clindamycin Increasing use of clindamycin for MRSA Increasing use of clindamycin for MRSA Importance of awareness of potential for Importance of awareness of potential for increased risk of treatment failure with positive D test: MRSA & MSSA MRSA & MSSA Rates of positive D test in S. aureus may Rates of positive D test in S. aureus may varies by age, region, time D-test erythromycin R/ clindamycin S MRSA & D-test erythromycin R/ clindamycin S MRSA & MSSA before reporting clindamycin susceptibility

Conclusion The high frequency of positive D test for MSSA isolates raises concern that clindamycin may fail to treat MSSA. The high frequency of positive D test for MSSA isolates raises concern that clindamycin may fail to treat MSSA. Clindamycin should be avoided as a treatment for patients infected by S. aureus exhibiting inducible resistance Clindamycin should be avoided as a treatment for patients infected by S. aureus exhibiting inducible resistance The proportion of S. aureus with in vitro inducible clindamycin resistance may vary by region, age group, and methicillin sensitivity The proportion of S. aureus with in vitro inducible clindamycin resistance may vary by region, age group, and methicillin sensitivity

Conclusion There has been a marked increase in the number of patients with infections that are unreceptive to clindamycin treatment. There has been a marked increase in the number of patients with infections that are unreceptive to clindamycin treatment. The large number of positive D test may explain the failure of clindamycin in treating the infected patients. The large number of positive D test may explain the failure of clindamycin in treating the infected patients. This study has demonstrated the importance of performing D test on MRSA Isolates. This study has demonstrated the importance of performing D test on MRSA Isolates.

Future studies Future studies Is it gender specific? Is it gender specific? Is it age specific? Is it age specific? Repeated infections? Repeated infections? Environment effects? Environment effects? Molecular mechanism of antibiotics resistance? Molecular mechanism of antibiotics resistance? Improving the D test. Improving the D test.

Acknowledgment Dr. Harrison Dr. Harrison BSC 661 BSC 661

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