Genetic Engineering and Stem Cells. * Restriction enzymes: * Have sticky ends or overhangs of DNA. * This helps to “glue” in the gene of interest in the.

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Presentation transcript:

Genetic Engineering and Stem Cells

* Restriction enzymes: * Have sticky ends or overhangs of DNA. * This helps to “glue” in the gene of interest in the right direction. * Must find a plasmid with the appropriate sticky ends that match your gene.

* Polymerase Chain Reaction * Taq polymerase * Nucleotides * Thermocycler *

* Gel Electrophoresis: * electrophoresis.html electrophoresis.html * Gene Chip: * tions/content/dnachips2.html tions/content/dnachips2.html * How could gene chip technology transform medicine as we know it?

* Learn how a mouse is cloned. * clickandclone/ clickandclone/ * Many copies of identical mice are cloned from mouse embryos. You can have essentially 1000 identical twins with the same genetics. * This has been used in science for many years.

* Epigenetic patterns already in place (how is DNA tagged to turn genes on and off?) * Transplantation process is costly. * Cells are genetically “older”. Telomerase has been deactivated.