1 Bio211 Laboratory 3 Tissues Muscle, Nerve, Integument

2 Tissues Tissues to be examined under the microscope –Muscle/Nervous Tissues (p. 109/117 Lab Manual) [TODAY] –Skin (Integument) (p. 123 Lab Manual) [TODAY] –Epithelial Tissue (p. 79 Lab Manual) [REVIEW] –Connective Tissue (p. 93 Lab Manual) [REVIEW] Refer to your –Wood's Lab Manual for pictures/guidance –Lab Guide (handout) for a checklist of tissues/structures you are responsible for Objectives –Learn to recognize each of the tissue types under the light microscope and how they are constructed –Provide at least one example of where each tissue is found in the body

3 Muscle Tissue Three types of muscle tissue –Skeletal (slide #21) –Cardiac (slide #24) –Smooth (slide #23 – histological preparation) Slide #22 is isolated smooth muscle (cytological preparation); look at this also See or Fig 9.1 (p.110) in Wood’s Lab Manual or Table 10.1 (p.75) in Hole’s Lab Manual for Muscle Tissue Characteristics

4 Skeletal Muscle Major characteristics –Contractile –Voluntary –Striated –Multinucleated –Unbranched Major Functions –Voluntary movement –Heat generation

5 Skeletal Muscle Notice that this tissue is: Striated, multinucleated, non-branched

6 Cardiac Muscle Major characteristics –Contractile –Involuntary –Striated –Single nucleus –Branched –Intercalated discs Major Function –Pump blood

7 Cardiac Muscle Notice that this tissue is: Striated, mononucleated, branched, and has intercalated disks

8 Smooth Muscle Major characteristics –Contractile –Involuntary –NOT Striated –Single, centrally located nucleus –Unbranched Major Function –Controls size/shape of organs –Constricts blood vessels

9 Smooth Muscle Notice that this tissue is: Not striated, mononucleated, and non-branched Cytology Histology

10 Nervous Tissue (slide #26) Major characteristics –Mononucleated (usually central) –Many cytoplasmic extensions –Usually surrounded by small, glial cells Major Functions –Transmission of nerve impulses –Sensory reception

11 Nervous Tissue

12 Integumentary System Things you should do for Integument… –FIRST: Label the diagram 11.8 in your Wood’s Lab manual (page 132) and become familiar with the structures –SECOND: Examine slide #53 and identify and be able to recognize the following for the lab exam Layers of the skin; epidermis, dermis, subcutaneous Sebaceous glands Sweat glands Hair follicles

13 Integumentary System

14 Integumentary System From: ages/Integumentary/Integum.htm#sebaceous

15 Integumentary System From: ages/Integumentary/Integum.htm#sebaceous

16 Integumentary System From: /Integumentary/Integum.htm#sebaceous Sebaceous glands Hair follicles

17 Integumentary System From: ages/Integumentary/Integum.htm#sebaceous

18 Integumentary System From: ages/Integumentary/Integum.htm#sebaceous Sweat gland

19 Light Microscope View of Sweat Glands From: Martini, Anatomy & Physiology, Prentice Hall, 1998

20 Review Muscle Tissue –Skeletal (Striated, multinuclear, unbranched) –Cardiac (Striated, mononuclear, branched, intercalated disks) –Smooth (Not striated, mononuclear, unbranched) –Summary Figure 9.1, page 110 in Wood's Lab Manual Nervous Tissue –Central nucleus –Many cytoplasmic processes –Usually surrounded by smaller neuroglial cells Integument –Layers of skin –Accessory structures

21 What you should do in lab today… Using the microscope and the slide boxes –Look at the slides listed on your Laboratory Guide (handed out in week 1) for: Muscle tissue (skeletal, cardiac, smooth) Nervous tissue Skin; the layers and its accessory organs (sebaceous glands, hair follicles, sweat glands) – see microscopes at front of lab BE SURE you can –recognize each of the tissue types listed in your Laboratory Guide (know the CHARACTERISTICS, not colors!) –Identify the major structures on the slides (cell nuclei, intercalated disks, striations, layers of epidermis, etc.) –Give an example where each of the tissues is found in the body

22 What you should do in lab today… Use Wood's Laboratory Manual as a guide to the things you should look for in each tissue Review all material from labs 1 – 3 for upcoming lab exam Test your lab partners to make sure you each can recognize the material from lab and know the structures involved, then come up and I will test you.

23 Format of Lab Exam 1 There will be 25 stations, a total of 53 questions The questions will be based on –Photographs, Diagrams (labeling) You will initially sit at ANY station You will rotate through the stations When I see everyone turn their answer sheets over, you will proceed to the next station Grades will be posted on Blackboard (private) YES, SPELLING COUNTS! (-1 pt/2 errors) See your Lab Exam 1 Study Guide

24 Online Quizzes Look under the Laboratory Materials on the gserianne.com Web site (NOT BB Vista) Don’t forget to do these! The quizzes will give you practice answering the kinds of questions you’ll have on the lab exam. Keep your Study Guide handy so you don’t forget to study something (that may NOT be on the quizzes) Quizzes are not graded

25 For next regular lab… Our Bones labs (3) –Will be VERY demanding –PLEASE read ahead and label as many diagrams in your lab book as you can BEFORE coming to lab to do the hands-on work! For Bone overview and Bones of the Skull –Read Exercises 13 &14 (Activity 1-4) in Wood's Lab Manual –Label the Exercise diagrams ahead of time –See gserianne.com Web site for some skull practice