Revolutionising DNA analysis in major crime investigations The Investigator Conferences Green Park Conference Centre May, 2014 Aylesbury, Buckinghamshire.

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Presentation transcript:

Revolutionising DNA analysis in major crime investigations The Investigator Conferences Green Park Conference Centre May, 2014 Aylesbury, Buckinghamshire Mark W Perlin, PhD, MD, PhD Cybergenetics, Pittsburgh, PA Cybergenetics ©

Commonwealth v. Lyons homicide: DNA mixture evidence Victim's blood spatter pulsating spray from severed carotid artery 75% victim 25% other

DNA molecule

Copy DNA Copy intact DNA

Normal DNA signal

Degraded DNA Can’t copy broken DNA

Longer molecules copy less With degradation, a longer DNA molecule has a greater chance of having a break

DNA decay curve DNA size Observed DNA With degradation, a longer DNA molecule makes fewer DNA copies

Degraded DNA signal

Degraded DNA Mixture Degraded other DNA Victim DNA

Match statistic comparison Human Computer 42,000 9,500,000,000,000

Computer Interpretation of Quantitative DNA Evidence Commonwealth v. Lyons June, 2011 Reading, PA Mark W Perlin, PhD, MD, PhD Cybergenetics, Pittsburgh, PA Cybergenetics ©

DNA evidence interpretation Evidence item Evidence data LabInfer Evidence genotype Known genotype 10, 50% 11, 30% 12, 20% 10, 12 Compare

Computers can use all the data Quantitative peak heights at locus D8S1179 victim other

People may use less of the data Over threshold, peaks become allele events. Under threshold, alleles vanish. All-or-none allele peaks; ignore victim genotype Threshold

How the computer thinks Consider every possible genotype solution victim other Explain the peak pattern Better explanation gives a higher probability

Evidence genotypes victimother

DNA match information Probability(evidence match) Probability(coincidental match) How much more does the suspect match the evidence than a random person? 6x

Is the suspect in the evidence? A match between the suspect and the evidence is 9.46 trillion times more probable than coincidence. Yes, with a reasonable degree of scientific certainty, evidence item Q9 contains DNA from the genotype of suspect item K2.

Is the victim in the evidence? A match between the victim and the evidence is 1.27 quintillion times more probable than coincidence. Yes, with a reasonable degree of scientific certainty, evidence item Q9 contains DNA from the genotype of victim item K1.

Getting Started

Questions?