1 The Detection of Fertility Hormones via Antibody Immobilization and Electrochemical Impedance Spectroscopy Student: Stephanie Maxwell Mentor: Dr. Jeffrey.

Slides:

Advertisements

Similar presentations

The menstrual cycle  Menstruation: the condition in female when the lining of the uterus breaks down and blood with cells pass out of the vagina during.

Advertisements

Female Hormonal Cycle or the MENSTRUAL CYCLE MENSTRUAL CYCLE Normal cycle length is considered 28 days, however it can range from days divided.

Menstrual Cycle Key words

Menstrual Cycle.

Stage 1 – Menstruation Starts on Day 1 – lasts 3-7 days Hormones – travel in blood to target tissue – ALL 4 hormones at lowest levels Ovary – – Follicle.

 Produced by endocrine glands  Male endocrine glands = Testes  Female endocrine glands = Ovaries.

Role of Hormones in Menstrual Cycle Anton, Barbara, Gabi.

Understanding Ovulation

Mr. Chapman Biology 30 Birth Control Pills – How They Work.

I N V ITRO F ERTILIZATION By, Lindsey New. In Vitro Fertilization or IVF is when eggs and sperm are combined outside the body in a laboratory and then.

Ovulation and Menstruation. Function of Menstrual Cycle Uterine walls thickens with rich blood supply in preparation for a fertilized egg If fertilization.

Female Reproductive System

 Follicle Stimulating Hormones (FSH) and Luteinizing Hormone (LH)- released in the pituitary gland.  Estrogen- Development of the female secondary sexual.

Sexual Reproduction in the Human (Extended Study).

MENSTRUAL CYCLE. Major stage of puberty (between the ages of 8 and 13) From the beginning of one period to the beginning of the next takes about 28 days.

The Menstrual Cycle.

Menstrual Cycle.

 The menstrual cycle is approximately a 28-day cycle which is completed in preparation for a human female to reproduce.

Menstruation IF fertilization does NOT take place Menstrual cycle – a series of changes controlled by hormones that help prepare the female uterus for.

WALT ; Describe how the menstrual cycle works All can identify the 4 hormones involved. Most can describe how they regulate the menstrual cycle Some can.

Do Now # 7 2/20/13 O 1.Why is it important to track your period? O 2. How do you track your period or know when you are close to getting your period?

The Menstrual Cycle LO:

Menstrual Cycle Regulation

Human Reproductive System. The Human Reproductive System Male sperm cells are produced in the testes Female eggs are produced in the ovaries At about.

By Johana Moreno Andrea Slobodien. Also known as the Menstrual Cycle Recurring cycle of physiological changes that occur in reproductive-aged woman Overt.

Human Reproduction 6.6.

The process of a male gamete (sperm) fertilizing a female gamete (egg or ovum)

By: McKenzie Gray and Bethany Sprauer. What kind of hormone is it? They are a group chemically similar to steroids A series of chemical reactions spurred.

The Menstrual Cycle.  Releasing of one egg (ovulation) every month from the ovaries  4 steps: Preparing the egg Releasing egg Preparing the endometrium.

HHD The Menstrual Cycle

Sexual Reproduction in the Human

Misconceptions about Conception Menstrual cycle is not always 28 days. Ovulation does not always occur on day 14. It is true that there is a fixed amount.

THE MENSTRUAL CYCLE 1 Resources

The Artificial Control of Fertility L.O: To understand how hormones are used to prevent pregnancy and increase fertility.

Sponge: Set up Cornell Notes on pg. 75 Topic: 22.2 Male and Female Sex Hormones Essential Question: How do hormones play a key role in the menstrual cycle?

By Funmi Agbaje.  Yam is a versatile vegetable belonging to Discoreaceae family  Starchy, tuberous root of tropical tree  They are a rich source of.

Today Spermatogenesis Oogenesis Hormonal control of menstrual cycle Fertilization.

Menstrual Cycle. Interaction between the endocrine and reproductive systems. From the Latin “mensis” meaning “month”

1 The Monthly Cycle Book Resources

Emergency Contraceptive Pills (ECPS) Click to Begin.

The Female Reproductive System Science 9. Secondary Sexual Characteristics Mammalian females have a vital role in reproduction Because fertilization is.

Female Reproductive Hormone Cycles. The Human Female Reproductive System The ovaries are where meiosis occurs and where the secondary oocyte forms prior.

Aim: How can hormones influence the reproductive cycles in humans? Do Now: Using the diagrams below, which structures produce hormones? What hormones are.

Biology 12 THE FEMALE MENSTRUAL CYCLE.  The menstrual cycle is the term for the physiological changes that can occur in fertile women for the purposes.

Label the picture: Ovary, Egg, Endometrium, Cervix, Uterus, Fallopian.

DHQ – Lesson 1 If you had to take a quiz on the female reproductive system, what would you struggle with?

Male and female sex hormones

Female Reproductive Cycle Or Menstrual Cycle

Understanding Ovulation

Menstrual Cycle and Fertilization

Menstrual Cycle Noadswood Science, 2016.

Menstrual Cycle From day one of a female’s period to day one of her next period.

Sexual Reproduction in the Human

Understanding Ovulation

Understanding Ovulation

Female Reproductive System

Menstruation IF fertilization does NOT take place

Menstruation IF fertilization does NOT take place

MENSTRUAL CYCLE.

Recap questions chapter 8b

Female Reproductive System

Introduction: Menstrual Cycle

The Menstrual Cycle.

1. FSH: Follicle-stimulating hormone; and LH: luteinizing hormone

The Female Menstrual Cycle

Sexual Reproduction in the Human

The Reproductive system

Presentation transcript:

1 The Detection of Fertility Hormones via Antibody Immobilization and Electrochemical Impedance Spectroscopy Student: Stephanie Maxwell Mentor: Dr. Jeffrey La Belle

2 The Objective:

3 WHY? More than 500,000 pregnancies each year end in miscarriage (occurring during the first 20 weeks). Over six million women in the United States struggle with becoming or staying pregnant. Hormone levels are constantly changing, and at times drastically. Pregnancies among women aged >40 years are associated with more non-severe complications, more premature births, more congenital malformations and more interventions at birth.

4 Estradiol Molecule: Estradiol is a hormone associated with triggering responses that initiate ovulation Progesterone Molecule: Progesterone is a steroid hormone, and it plays an important role in the preparation for and maintenance of pregnancies Follicle Stimulating Molecule: The amount of FSH varies throughout a woman's menstrual cycle and is highest just before she releases an egg Target Hormones

5 Methods: Immobilization

6 Methods: Cyclic Voltammetry INPUTOUTPUT

7 Methods: Electrochemical Impedance Spectroscopy

8 Calibration Curve Calibration curve created from the average of 7 different concentration gradients performed through EIS. Impedances were taken at the specific binding frequency (371Hz).

9 Interferent Testing Calibration curves created after adding a concentration gradient of interferents to a concentration gradient of progesterone.

10 Calibration in blood The effect of blood percentage on the slopes and R squared values of calibration curves of progesterone at 371Hz

11 Conclusions The optimal binding frequency of progesterone was found (371Hz) and a linear relationship between impedance and log concentration was determined This relation is reproducible (R^2=0.99, n=7) The response decreases with an increase in blood percentage The relation holds true with Estradiol acting as an interferent; however a lot of noise is produced with the addition of Albumin

12 Next steps Non-target testing will also be completed to further prove specificity of the antibody Selectivity Sensitivity Combining and analyzing data from all targets

13 Electrochemical Impedance Spectroscopy ν1ν1 ν2ν2 ν3ν3 Target Concentration Impedance (Z) Simultaneous detection of multiple hormones associated with pregnancy and/or fertility Progesterone FSH Estradiol Final Goal

14 Thank you!