The Microscope.

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Presentation transcript:

The Microscope

Microscopes Most important tool for biologists Types: Dissecting Microscope, Light Microscope, & Electron Microscope

What does a microscope do? Enlarges specimens so we can see details

How does it work? Light is shined through the (stained) specimen and is magnified with various lenses

Compound Light Microscope Structures Ocular Lens (eyepiece) Topmost lens through which an object is viewed. Usually 10x magnification

Structures … Revolving Nosepiece Rotating piece below the ocular lens that hold the objective lenses

Structures … Arm Supports the upper parts of the microscope and provides a carrying handle

Structures … Objective lenses: Scanning objective holds 4X lens used to view the whole slide. Lower Power Objective holds 10X lens used to view the object in greater detail Higher Power Objective holds 40X lens used to view the object in even greater detail

Magnification To find the total magnification you must multiply the ocular lens by the objective lens you are using For example: 10x X 40x has a total magnification of 400X

Structures … Coarse Adjustment Knob – used to bring an object into approximate focus used ONLY with scanning objective!

Structures … Fine Adjustment Knob used to bring object into final focus Used ONLY with Lower & High Powered Objectives!

Structures … Diaphragm Controls the amount of light used to view the object

Structures … Light Source A lamp that directs a beam of light up through the objective

Structures … Base Flat surface of the microscope that rests on the lab table Also used for carrying the microscope, along with the arm

Structures … Stage Holds and supports microscope slides

Structures … Stage clips Holds microscope slides in place