NHMFL User Committee Meeting NMR/MRI Subgroup October 2 nd 2009 Update from The Advanced Magnetic Resonance Imaging & Spectroscopy Program at the University of Florida and The NMR Spectroscopy & Imaging Program at Florida State University

NHMFL User Committee Meeting NMR/MRI Subgroup October 2 nd 2009 Agenda 11:00 NMR/MRI Program Overviews, Tim Cross Joanna Long 11:45 Lunch 12:30 Tallahassee Presentations Fengli Zhang Sam Grant Zhehong Gan Bill Brey 14:00 HTS NMR Magnet Prospects 14:30 Coffee Break 14:45 Gainesville Presentations Tom Mareci Steve Blackband Aaron Dossey 16:15 Discussion of Priorities

User Statistics from NMR/MRI NHMFL Activities ICR, NMR, EMR, AMRIS and Geochemistry provide the majority of the chemical & biological research user programs ICR, NMR, EMR, AMRIS and Geochemistry provide the majority of the chemical & biological research user programs and represent 25-45% of the Magnet Lab user productivity and represent 25-45% of the Magnet Lab user productivity »» Research Reports ( ): NMR/MRI (UF & FSU)195 ICR81 EMR134 total ~1/2 are chem/bio67 Geochemistry75 total - represents 26% of all NHMFL reports418 »» Research Reports for 2008: NMR/MRI (UF & FSU)85 ICR15 EMR39 total ~1/2 are chem/bio20 Geochemistry22 total - represents 38% of all NHMFL reports 144

User Statistics from Chem/Bio NHMFL Activities ICR, NMR, EMR, AMRIS and Geochemistry ICR, NMR, EMR, AMRIS and Geochemistry »» Users for 2008: NMR/MRI (UF & FSU)257 ICR97 EMR99 total ~1/2 are chem/bio50 Geochemistry46 total represents 46% of all NHMFL users450 »» Publications for 2008: NMR/MRI (UF & FSU)77 ICR17 EMR47 total ~1/2 are chem/bio24 Geochemistry9 total represents 30% of all NHMFL pubs 127 represents 46% of all NHMFL User Facility Pubs

UF/FSU NMR/MRI News and Collaborative Efforts Art Edison agreed to serve the NHMFL as Associate Director for Chemistry and Biochemistry This summer he led an effort to write a proposal for a 950 MHz spectrometer focusing on 13 C direct detection at high fields with both cryo and HTS probes - the latter to be jointly developed by Bill Brey and Agilent, building on the previous collaboration between Bill Brey and Rich Withers, formerly of Bruker. Funding for a new HTS probe has been achieved represents a similar collaboration to that described above, but here for a higher mass sensitivity probe at 1.5 mm with enhanced filling factor. The External Advisory Committee met in August and recommended that the vacant chief scientist position be filled with a Chem Bio researcher with major NIH funding.

FSU NMR Spectroscopy and Imaging Mission »» Multi-Frequency RF Probe Development »» Low-E RF Probes »» HTS Probe Development »» Additional Probe Hardware for Unique Capabilities »» Magnetic Field Stabilization Hardware »» Theoretical Spin Physics »» Novel Pulse Sequence Development »» Web-based Metabolomics Tools »» Experimental Protocols »» Technology and Science Drivers for Novel and Ultra High Field Magnets User Driven Unique Technology Development for High Field NMR Spectroscopy and Imaging User Driven Development of Unique Applications for High Field NMR Spectroscopy and Imaging »» Inorganic Chemistry and Materials Chemistry »» Biophysics and Biochemistry »» Drug Screening and Metabolomics »» Anatomy and Biomedical Science »» Spin Physics »» Macromolecular Dynamics and Structure

FSU Facility Highlights from ‘08 Installed Bruker Biospin Avance III console for the 900 and the Avance I 900 console will replace the current DRX console on the WB600 - this was part of a $3.2M contract for NMR instrumentation to FSU. For the 900 new console signal generation units and receivers, but with the previous RF power and gradient amplifiers as well as previous preamplifiers. Up to date software for the 900 and WB600. » state of the art capability for solids and imaging on the 900 and much improved capability for the WB600 - both WB 600 instruments will have Avance I consoles New WB % for Prof. Anant Paravastu and 50% for the NMR User facility - primarily for MAS solids. A 1.3 mm 60kHz MAS probe will be delivered with the instrument

2009 Planned Proposals for NMR User Program Proposal to NIBIB for a BRP in support of solid state NMR and and Na + imaging: “Novel Technology for Biomedical Research in Ultra-High Magnetic Fields” - letter requesting permission to submit proposal will be sent out next week. Proposal to be submitted 2/09. PI will be Cross S10 Proposal to the NCRR ‘High-End NMR’ program for a 900 MHz console - to be submitted 1/09. PI will be Sam Grant HTS 3mm probe for structural biology - to be submitted in late ‘09. PI will be Brüschweiler Proposal after 12 months of negotiations with NIBIB was refused for lack of interest in High Field Imaging and SSNMR. Plan involved lease/purchase arrangement and University refused to permit us to submit it - FSU funded $2.5M and the NHMFL provided $0.7M for console upgrades. Proposal led by Art Edison for 950 MHz with 3 mm HTS probe submitted for High-End Instrumentation to NCRR Follow up on plans presented last year

2009 Planned Proposals for NMR User Program NIH proposal (mechanism undefined) for design/ construction of a 30 T high homogeneity HTS magnet - to be submitted in late ‘09 NSF MRI proposal for the 36 T SCH console - to be submitted 1/10 Proposal led by Bill Brey for SCH console submitted as an MRI Proposal to NSF After extensive deliberation it appears that we should obtain more preliminary HTS magnet results - 32 T HTS cold bore magnet for physics has been funded by NSF MRI. Follow up on plans presented last year $9M Program Project Grant funded with UCSD, U. Alabama, Burnham Inst., & Harvard - validation of membrane protein drug targets - search for ligands that bind (initial drug screening) - structural characterization of 10 TB membrane proteins - development of triple resonance low-E probes for high fields - development of solid state NMR drug screening probes Additional funding efforts

Moving Forward - Continuing Facility Enhancement in Tallahassee - setting priorities Last few years we pointed out that much of our instrumentation is seriously aging, some progress has been made. » UWB new console delivered in ‘09 » VNB needs a high power RF amplifier - will order Fall ‘09 » SB replace with a solution NMR centerpiece Proposal submitted » SB replace the 10 yr. old console - (along with 950 purchase) » WB 600a - an Avance I system - not currently a top priority » WB 600b - to be upgraded to Avance I in ‘09 » SB 500 w/cryo - receives little demand » (WB 500) - to be delivered in ‘09 » WB an Avance I console » SB staging instrument for the Keck magnet with TecMag console » WB important staging instrument - 14 year old DRX console » 36 T SCH - proposal submitted summer ‘09

Moving Forward - Continuing Facility Enhancement in Tallahassee - setting priorities - May ‘09 report to GSB Emergency Needs (list one item) Replacement of the 720 MHz instrument (950 MHz proposal - no resources if this is not funded) Top Priority Needs (list three items to maintain existing user program without growth) Spectroscopist scholar scientist for the 950 MHz instrument if funded Series Connected Hybrid Console if not funded (MRI R 2 proposal - no resources if this is not funded) Expense and Equipment for RF development and maintenance Middle Priority Needs (list three items - necessary growth to maintain premier position) Jason Kitchen - RF engineer - temporarily funded on NMR account without budget Series Connected Hybrid Scholar Scientist Console for 600 MHz instrument (should be done in concert with 720 replacement) Emergency Needs (list five items - visionary growth in user program or strategic planning) Development of DNP Capabilities Animal Resource Technician (2 years of support from Grant’s start up) Software Application Engineer to facilitate software disbursement Replacement of two WB600 and 830 MHz consoles We would like to have your advice on these or other priorities