Ex. 15 Oxidase Test Objectives: ?? Cytochrome oxidases help with transfer of electrons from cytochromes to oxygen.

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Ex. 15 Oxidase Test Objectives: ?? Cytochrome oxidases help with transfer of electrons from cytochromes to oxygen.

Electron Transport Phosphorylation Textbook Fig 5.16

Oxidase Test Determines presence of certain cytochrome oxidases Use whenever Gram-neg. bacteria have to be identified When test reagent gets oxidized it changes color. Oxidase test is positive if color change within 15 seconds!

Only use on young cultures Table 15-1: Oxidase Test Results and Interpretation ResultInterpretation Symbol Dark blue within 15 secondsCytochrome c oxidase is present+ No color change to blue or blue after 15 seconds Cytochrome c oxidase is absent Oxistrip

Materials needed per table:  Oxidase reagent (bottle with dropper)  Small pieces of paper towel  sterile Q-tips (2 per student) Pure slant culture needed per table: 1.E. coli 2.Pseudomonas aeruginosa Each student should individually do the oxidase test for both bacterial species.