TOPIC: Microscope AIM: What are the parts of a microscope?

Microscope Instrument that produces a larger image of an object (specimen) using lenses

Lens Piece of curved glass Causes light rays to come together or spread apart Magnify an image

Uses 1 lens and light to magnify the specimen Simple Microscope Uses 1 lens and light to magnify the specimen

Uses 2 lenses & light to magnify specimen Need stain (dye) to see it Compound Light Microscope Uses 2 lenses & light to magnify specimen Need stain (dye) to see it

Blood

Cheek Cells 40x

Onion Cells 10x

PARTS OF A MICROSCOPE

1. eyepiece Ocular lens (usually 10x) Lens you look through

2. Objective lenses Contains lenses Low, medium, high power High Power Low Power High Power

Low power objective = used to locate the specimen on the slide Larger field of view (See more of the entire object) High power objective = More magnification Smaller field of view(see less of the entire object)

Holds & rotates obj. lenses 3. Nosepiece Holds & rotates obj. lenses

Connects eyepiece to nosepiece 4. Body Tube Connects eyepiece to nosepiece

Supports body tube 5. Arm

Bottom = support 6. Base

Where slide is placed 7. Stage

Hold slide in place 8. Stage Clips

Illuminates specimen, provides light 9. Mirror/ light

Controls the AMOUNT of light used 10. Diaphragm Controls the AMOUNT of light used

Larger focusing knob 11. Coarse Adjustment Knob

For focusing under LOW POWER ONLY If used under high power, objective lens and/or slide can break

Focusing for High Power Sharpens image Focusing for High Power 12. Fine Adjustment Knob

Most powerful microscope Electron microscope: magnifies up to 1 million times Uses beam of electrons instead of light

Fly

Spider

Termite head

Tapeworm

Blood

SEM Scanning Electron Microscope Produces real, 3D image Only scans surface magnifies 20x - to 200,000x SEM

TEM Transmission Electron Microscope Produces 2D image of thinly sliced organism magnifies 1,000x -1,000,000x

View living specimens without staining Phase Contrast Microscope View living specimens without staining

Light microscope Phase contrast microscope