 Histology  Tissues - animal, human, plant  Sources - medical, veterinary, research  Grossed, fixed, processed, embedded, sectioned, and stained  Important to the scientific investigation process  To establish and confirm patient diagnosis  For data compilation for researchers. What is histology?

 Grossing  Tissue is cut to appropriate size & thickness  Fixation  Stabilizes tissue proteins  Prevents self-destruction What is histology?  Preserves cells/tissue constituents  SSHS offers a range of fixatives  Non-buffered formalin  Bouins’ solution  Zinc formalin

 Processing  Tissue runs overnight in a tissue processor  Tissue goes through series of reagents  Dehydrates tissue  Transition step  Fills with hot fluid wax What is histology?

 Embedding  Tissue removed from processor cassette  Aligned in a mold  Molten paraffin poured over  Cooled to harden What is histology?

 Sectioning  Use microtome  Cut into microscopically thin strips  Floated on a water bath to smooth out wrinkles  Sections arranged on microscope slides What is histology?

 Staining  Slides are dried in front of a fan  Tissue is deparaffinized by heating in an oven  Slides are stained  Many different types of stains and staining techniques  Dependent on tissue type and what researcher/pathologist is looking for What is histology?

Saffron will provide histology services tailored to the unique needs of our research, veterinary, physician, hospital, and surgery center clients. The management team is known for high quality slides, reliable service, fast turnaround, and competitive prices. Mission Statement