Dalia kamal Eldien Mohammed. Material:  Sensitive balance  Flask  Measuring cylinder  Filter paper  Distilled water  Bunsen burner  Autoclave 

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Presentation transcript:

Dalia kamal Eldien Mohammed

Material:  Sensitive balance  Flask  Measuring cylinder  Filter paper  Distilled water  Bunsen burner  Autoclave  Sterile Petri dish  Bottle of media to be prepared

Method  Read the formula in the bottle of media, and count the amount of the powder according to the volume you want to prepare  Using the sensitive balance weight the media  Add the accurate amount of distilled water  Dissolve the content by heating in BB  Sterilize the media in autoclave 121C for 10 minutes, or according to the instruction in the bottle  Dispense in sterile petri dishs

 According to the formula Prepare  100 ml of 10% blood agar  100 of CLED  100m kilglar iron agar

 Write the aim, principle, method and the uses in microbiology lab, for the following instrument:-  Autoclave  Hot air oven  Incubator  Microscope  Water bath  Safety cabinet