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Presentation transcript:

m/blog/science- scope/three-parent- embryo-could-produce- disease-free-designer- babies/1149

mom again? Embryos can be made with nuclear and mitochondrial DNA from multiple parents.ANDY WALKER, MIDLAND FERTILITY SERVICES/ SCIENCE PHOTO LIBRARY

FIG. 1. Somatic cell nuclear transfer using human and animal oocytes. (A) Stages of nuclear transfer. Left panel: visualization of mitotic spindle complex using Oosight™ spindle imaging system, arrow indicates the location of the spindle complex; middle panel: removal of the spindle complex; right panel: injection of somatic cell nucleus. (B) Control intraspecies blastocysts generated using mouse, rabbit, and bovine oocytes. (C) Development of interspecies cloned embryos and a human IVF embryo. (D) genomic DNA and mitochondrial DNA analysis of iSCNT embryos. In each group, top panel: animal genomic DNA, middle panel: animal mtDNA; bottom panel: human mitochondrial DNA. Bp—DNA size ladder; T1— human total DNA; T2—animal total DNA; NC—negative control; numbers correspond to different blastomere samples analyzed.

FIG. 3. Hierarchical clustering of genes that are most differentially expressed in the donor somatic cells compared to the cloned embryos. Following median normalization and log transformation of the microarray data, an ANOVA analysis was performed on all 22 samples, and yielded 4838 differentially expressed genes (fold change threshold 4; p 0.005; with Bonferroni correction). Column 1: averaged global transcription profiles obtained from donor human somatic cells (n 3); column 2: normal human IVF embryos (n 6); column 3: human–human SCNT embryos (n 3); column 4: human–bovine iSCNT embryos (n 6); and column 5: human–rabbit iSCNT embryos (n 4). Red indicates upregulation; green indicates downregulation.

re-hype-about-three-parent-embryos.html

Expanded. New rules approved by British lawmakers increase the types of embryo research that are allowed. SCIENCE

Young Chung,1 Colin E. Bishop,2 Nathan R. Treff,3 Stephen J. Walker,2 Vladislav M. Sandler,1 Sandy Becker,1 Irina Klimanskaya,1 Wan-Song Wun,5 Randall Dunn,4 Rebecca M. Hall,5 Jing Su,3 Shi-Jiang Lu,1 Marc Maserati,1 Young-Ho Choi,6 Richard Scott,3 Anthony Atala,2 Ralph Dittman,5 and Robert Lanza1,2 1) Advanced Cell Technology, Worcester, Massachusetts. 2) Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, North Carolina. 3) Reproductive Medicine Associates of New Jersey, Morristown, New Jersey. 4) Fertility Specialists of Houston, Houston, Texas. 5) Stem Cell Source, LLC, Houston, Texas. 6) Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas.