DO NOW: Take out your microscope YELLOW packets from last week. In your NOTES section of your binder, please list EIGHT differences between these two pictures.

Stare at the red dot on the girl’s nose for 30 seconds. Turn your eyes to a plain surface. Blink repeatedly and quickly.

Using a Compound Light Microscope Directed Reading

1.) The magnifies the image x. 2.) The low- magnifies the image x. Using the Microscope 1.) The magnifies the image x. 2.) The low- magnifies the image x. 3.) The high- magnifies the image either x or x. ocular lens 10 power objective 10 power objective 43 40

Using the Microscope 4.) The holds the objective and can be turned to change from one to the other. 5.) The maintains the correct distance between the and .   6.) The moves the body tube and down to allow focusing of the . revolving nosepiece magnification body tube ocular lens objectives coarse - adjustment knob image up

7.) The moves the body tube to bring the image into focus. Using the Microscope fine- adjustment knob 7.) The moves the body tube to bring the image into focus.   8.) The supports a slide. 9.) hold the slide in place for . slightly sharper stage Stage clips viewing

10.) The controls that amount of coming through the stage. Using the Microscope 10.) The controls that amount of coming through the stage. 11.) The light sources provides a for viewing the . 12.) The supports the body . diaphragm light light slide arm tube

Using the Microscope 13.) The supports the . 14.) Inclination joint (hinge): Allows the upper part of the microscope to be tilted back to view the specimen while seated. base microscope

Proper Use of the Compound Light Microscope 1.) How would you carry a microscope? With your left hand With your right hand With both hands

Proper Use of the Compound Light Microscope 2.) How many centimeters should the microscope be from the edge of a table? 1 cm 5 cm 10 cm

Proper Use of the Compound Light Microscope 3.)  What power objective should you ALWAYS begin working with? Low Medium High

Proper Use of the Compound Light Microscope 4.)  What must be adjusted to get the microscope into low power? Revolving objective Revolving nosepiece Revolving ocular lens

Proper Use of the Compound Light Microscope 5.) Once you place a prepared slide over the hole in the stage, what secures the slide? A rubber band Your finger Stage clips

Proper Use of the Compound Light Microscope 6.) If the diaphragm is moved, what gets adjusted? The height of the stage The amount of light The objective lens

Proper Use of the Compound Light Microscope 7.) Once you are looking at the stage from eye level, you should slowly turn the: Fine adjustment knob Medium adjustment knob Course adjustment knob

Proper Use of the Compound Light Microscope 8.) You should NEVER allow the objective to touch the slide True False Sometimes it can

Proper Use of the Compound Light Microscope 9.) While looking through the ocular lens, you should turn the coarse adjustment knob to raise the low-power objective so that the image is: Focused Blurred Not visible

Proper Use of the Compound Light Microscope 10.) Once the object is focused, what sharpens the image? Fine adjustment knob Medium adjustment knob Coarse adjustment knob

Proper Use of the Compound Light Microscope 11.)  When you are in high power you should always use the coarse adjustment knob? True False Sometimes

Proper Use of the Compound Light Microscope 12.) When you are finished using the microscope, you should remove the slide and clean the ocular and objective lens with what type of paper? Lens paper Sand paper Construction paper

Proper Use of the Compound Light Microscope 13.) When you are all finished using the microscope, you should always: Return it to the storage area Leave it where it is Throw it away

Making a Wet Mount Slide 1.) Use sand paper to clean a glass slide and a coverslip. LENS

Making a Wet Mount Slide 2.)  Place the specimen you wish to observe on the left of the slide. CENTER

Making a Wet Mount Slide 3.)  Using a medicine dropper, place 5 drops of water on the specimen. ONE

Making a Wet Mount Slide 4.)  Hold the coverslip at the edge of the water at a 90 degree angle to the slide. Make sure the water runs along the middle of the coverslip. 45 & EDGE

Making a Wet Mount Slide 5.)  Lower the coverslip quickly to avoid trapping air bubbles. SLOWLY

Making a Wet Mount Slide 6.)  Water might evaporate from the slide as you work. Remove more water to keep the specimen fresh. ADD

Making a Wet Mount Slide 6a.)  Place the tip of the medicine dropper next to edge of the coverslip. Add 4 drops of water. A (ONE)

Making a Wet Mount Slide 6b.)  Remove excess water from the slide by using the center of a paper towel as a blotter. CORNER

Making a Wet Mount Slide 6c.)  Lift the coverslip to add or remove water. DO NOT