AP Biology Ch. 20 Biotechnology.

Slides:



Advertisements
Similar presentations
DNA Technology & Genomics
Advertisements

DNA Technology & Genomics
Recombinant DNA Technology
Biotechnology Chapter 11.
Biotechnology Guess the lamb’s name
Biotechnology Chapter 20.
Manipulating the Genome: DNA Cloning and Analysis 20.1 – 20.3 Lesson 4.8.
Chapter 20 Reading Quiz Genes from two different sources that are combined result in ____. Where are “sticky ends” found? What structures, naturally found.
DNA TECHNOLOGY AND THE HUMAN GENOME. MOST DNA TECHNOLOGY IS NATURALLY OCCURING PHENOMENA THAT WE MANIPULATE TO SERVE OUR CURIOUSITY AND INTEREST – BACTERIAL.
DNA TECHNOLOGY DNA recombination or genetic engineering is the direct manipulation of genes for practical purposes.
DNA Technology and Genomics. Recombinant DNA n Definition: DNA in which genes from 2 different sources are linked n Genetic engineering: direct manipulation.
Biotechnology Packet #26 Chapter #9. Introduction Since the 1970’s, humans have been attempted to manipulate and modify genes in a way that was somewhat.
Biotechnology Techniques How to make Recombinant DNA Gel Electrophoresis PCR Summarize: What is this technique? Draw and label a diagram to show this technique.
Chapter 20: Biotechnology. Essential Knowledge u 3.a.1 – DNA, and in some cases RNA, is the primary source of heritable information (20.1 & 20.2)
Chapter 20~ DNA Technology & Genomics
DNA Technology n Now it gets real….. O.J. Simpson capital murder case,1/95-9/95 Odds of blood on socks in bedroom not being N. Brown-Simpson’s: 8.5 billion.
Chapter 20~DNA Technology & Genomics. Who am I? Recombinant DNA n Def: DNA in which genes from 2 different sources are linked n Genetic engineering:
AP Biology: Chapter 14 DNA Technologies
DNA Technology Ch. 20 Figure 20.1 An overview of how bacterial plasmids are used to clone genes.
Chapter 20 DNA Technology and Genomics
Gene Technology Chapter 16.
Chapter 20 DNA Technology. DNA Cloning  Gene cloning allows scientists to work with small sections of DNA (single genes) in isolation. –Exactly what.
AP Biology Chapter 20 DNA Technology.
Genetic technology. Some terminology Genetic engineering –Direct manipulation of genes for practical purposes Biotechnology –Manipulation of organisms.
Biotechnology Packet #12 Chapter #9. Introduction Since the 1970’s, humans have been attempted to manipulate and modify genes in a way that was somewhat.
Chapter 19 - Chromatin DNA PackagingDNA Packaging histone proteinhistone protein NucleosomeNucleosome ”beads on a string” basic unit of DNA packing”beads.
DNA Technology.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece.
Chapter 16 Gene Technology. Focus of Chapter u An introduction to the methods and developments in: u Recombinant DNA u Genetic Engineering u Biotechnology.
Ch. 20 Biotechnology. DNA cloning yields multiple copies of a gene or other DNA segment Gene cloning and other techniques, collectively termed DNA technology,
Chapter 20 Reading Quiz 1. Genes from two different sources that are combined result in ____. 2. Where are “sticky ends” found? 3. What structures,
Chapter 13 Table of Contents Section 1 DNA Technology
DNA Technology. Overview DNA technology makes it possible to clone genes for basic research and commercial applications DNA technology is a powerful set.
Biotechnology.
19.1 Techniques of Molecular Genetics Have Revolutionized Biology
DNA TECHNOLOGY AND GENOMICS CHAPTER 20 P
DNA Technology Chapter 11. Genetic Technology- Terms to Know Genetic engineering- Genetic engineering- Recombinant DNA- DNA made from 2 or more organisms.
Chapter 20 DNA Technology and Genomics. Viruses have restriction enzymes to attack and destroy invading viral DNA. Restriction enzymes cut DNA at specific.
By Melissa Rivera.  GENE CLONING: production of multiple identical copies of DNA  It was developed so scientists could work directly with specific genes.
Chapter 20: Terms to Know Genetic engineering Biotechnology
Biology 1060 Chapter 20 DNA Technology and Genomics.
KEY CONCEPT Biotechnology relies on cutting DNA at specific places.
GENETIC ENGINEERING CHAPTER 20
NOTES - CH 15 (and 14.3): DNA Technology (“Biotech”)
DNA Technology and Genomics
DNA Technology Ch. 20. The Human Genome The human genome has over 3 billion base pairs 97% does not code for proteins Called “Junk DNA” or “Noncoding.
Chapter 20 DNA Technology and Genomics. Biotechnology is the manipulation of organisms or their components to make useful products. Recombinant DNA is.
CH. 20 WARM-UP Share 3 things you are grateful for. Use your textbook (Ch. 20) to answer the following review questions. 1. What is recombinant DNA? 2.
Gene Technologies and Human ApplicationsSection 3 Section 3: Gene Technologies in Detail Preview Bellringer Key Ideas Basic Tools for Genetic Manipulation.
Copyright © 2005 Pearson Education, Inc. Publishing as Benjamin Cummings PowerPoint Lectures for Biology: Concepts and Connections, Fifth Edition – Campbell,
RECOMBINANT DNA DNA THAT CONTAINS DNA SEGMENTS OR GENES FROM DIFFERENT SOURCES. DNA TRANSFERRED FROM ONE PART OF A DNA MOLECULE TO ANOTHER, FROM ONE CHROMOSOME.
Biotechnology You Will Learn About… Transformation Cloning DNA Fingerprinting by Restriction Fragment Length Polymorphism (RFLP) What is the name of the.
DNA Technology & Genomics CHAPTER 20. Restriction Enzymes enzymes that cut DNA at specific locations (restriction sites) yielding restriction fragments.
Chapter 19 DNA Technology. Genetic Engineering Genetic engineering Genetic engineering the manipulation of genetic material for practical purposes the.
DNA Technology and Genomics
Chapter 20: Terms to Know Genetic engineering Biotechnology
DNA Technology Packet #27.
Additional DNA Technology AP Biology Ms. Day
DNA Technology and Genomics
DNA Tools & Biotechnology
DNA Technology Now it gets real…..
DNA Technology & Genomics
Chapter 20 – DNA Technology and Genomics
Forensic Evidence and Genetic Profiles
Chapter 14 Bioinformatics—the study of a genome
Recombinant DNA Technology
DNA Tools & Biotechnology
CHAPTER 20 DNA TECHNOLOGY.
Lecture #9 Date _____ Chapter 20~ DNA Technology & Genomics.
GENE TECHNOLOGY Chapter 13.
Presentation transcript:

AP Biology Ch. 20 Biotechnology

DNA Cloning DNA cloning and other techniques can be used to manipulate and analyze DNA and to produce useful new products and organisms. DNA cloning permits production of multiple copies of a specific gene or other DNA segment.

Restriction Enzymes to Make Recombinant DNA Bacterial restriction enzymes cut DNA molecules within short, specific nucleotide sequences to yield a set of double-stranded DNA fragments with single-stranded sticky ends. The sticky ends on fragments from one DNA source can base-pair with complementary sticky ends on fragments from other DNA molecules.

Using restriction enzymes and DNA ligase to make recombinant DNA

Cloning a Eukaryotic Gene in a Bacterial Plasmid A recombinant plasmid is made by inserting restriction fragments from DNA containing a gene of interest into a plasmid vector that has been cut open by the same enzyme. Gene cloning results when the recombinant plasmid is introduced into a host bacterial cell and the foreign genes are replicated along with the bacterial chromosome as the host cell reproduces.

Overview of gene cloning with a bacterial plasmid

Cloned genes, cont. A clone of cells carrying the desired gene can be identified with a radioactively labeled nucleic acid probe. Eukaryotic cells can also serve as host cells for gene cloning.

<< Nucleic Acid Probe < >

Cloning a human gene in a bacterial plasmid

Cloned Genes Cloned genes are stored in genomic libraries, which are collections of recombinant vector clones produced by cloning DNA fragments derived from an entire genome. A cDNA library is made by cloning DNA made in vitro by reverse transcription of all the mRNA produced by a particular kind of cell. Libraries of cDNA are especially useful for working with eukaryotic genes and for studying gene expression.

<< Genomic Libraries < >

Expressing Eukaryotic genes Several technical difficulties hinder the expression of cloned eukaryotic genes in bacterial host cells. The use of cultured eukaryotic cells as host cells and yeast artificial chromosomes (YACs) as vectors helps avoid these problems.

Polymerase Chain Reaction The PCR can be used to produce many copies of a specific target segment of DNA, using primers that bracket the desired sequence and a heat-resistant DNA polymerase.

Polymerase Chain Reaction << Polymerase Chain Reaction < >

Restriction fragment analysis Restriction fragment analysis detects DNA differences that affect restriction sites. Gel electrophoresis can separate DNA restriction fragments of different lengths. Specific fragments can be identified using Southern blotting, which uses labeled probes that hybridize to the DNA immobilized on a “blot” of the gel.

<< Gel Electrophoresis < >

<< Southern Blotting < >

Restriction Fragment Length Polymorphisms (RFLP) RFLPs are differences in DNA sequence on homologous chromosomes that result in restriction fragments of different lengths, which can be determined using Southern blotting. The thousands of RFLPs present throughout eukaryotic noncoding DNA can serve as genetic markers. RFLP analysis has many applications, including genetic mapping and diagnosis of genetic disorders.

<< RFLP Allele Analysis < >

<< RFLP Markers < >

Mapping Genomes Entire genomes can be mapped at the DNA level. The Human Genome Project is an international research effort that involves linkage mapping, physical mapping, and DNA sequencing of the human genome and the genomes of other organisms. An alternative approach starts with sequencing of random DNA fragments, relying especially heavily on computers to assemble the sequences. The human genome is thought to have 30,000 to 40,000 genes, fewer than what was once estimated.

Genome Size of Different Organisms << Genome Size of Different Organisms <

Sanger Method of Sequencing DNA << Sanger Method of Sequencing DNA < >

Alternative Sequencing Strategies << Alternative Sequencing Strategies < >

Gene Sequences Genome sequences provide clues to important biological questions; helping researchers find new genes, probe details of gene organization and control, and answer questions about evolution. DNA microarrays allow researchers to compare patterns of expression in different tissues and under different conditions.

<< DNA Microarray < >

Gene sequences, cont. Genomics is the study of entire genomes; proteomics is the study of proteins coded for by a genome. Single nucleotide polymorphisms (SNPs) provide useful markers for studying human genetic variation.

Practical Applications of DNA technology DNA technology is reshaping medicine and the pharmaceutical industry. Medical applications include diagnostic tests for genetic and other diseases, safer, more effective vaccines, and the prospect of treating or even curing certain genetic disorders. Pharmaceutical applications include the large-scale production of many new, and some previously scarce, drugs and other medicines.

<< Gene Therapy < >

DNA tech applications, cont. DNA technology offers forensic, environmental, and agricultural applications. DNA “fingerprints” obtained from RFLP or STR analysis of tissue found at the scenes of violent crimes provide evidence in trials. Such fingerprints are also helpful in resolving parenthood disputes.

<< DNA Fingerprints < >

DNA tech applications, cont. Genetic engineering can modify the metabolism of microorganisms so that they cab be used to extract minerals from the environment or degrade waste materials. In agriculture, transgenic plants and animals are being designed to improve food productivity and quality.

Using plasmid vectors in agriculture << Using plasmid vectors in agriculture < >

DNA Technology: Ethical and safety concerns Several U.S. government agencies are responsible for setting policies about and regulating recombinant DNA technology. The potential benefits of genetic engineering must be carefully weighed against the potential hazards of creating products or developing procedures that are harmful to humans or the environment.